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Experimental Study of Silver Based Nanostructures Biocompatibility for Tissue Engineering

Aghababaie Khouzani, Zahra | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 49175 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vossoughi, Manouchehr; Yaghmaei, Soheyla
  7. Abstract:
  8. Acceleration of healing process for crucial wounds has been remained a challenging issue and it is critical to improve treatments against infection during wound healing period. Among various antimicrobial agents, silver components has been extensively since they are resistant against a wide range of bacteria. In this study, we developed electrospun mats composed of Polycaprolactone (PCL) and Polyvinyl alcohol (PVA) loaded with major silver components using co-electrospinnig method. Chitosan- Ag nanoparticles was synthesized using chitosan via heating prior to electrospinning. Various amounts of Silver components including Ag+, Silversulfadiazine (SSD) and Chitosan- Ag NPs were added to PVA to fabricate wound dressings. Ultra-Violent irradiation was used to reduced Ag+ and form AgNPs in Ag+ containing nanofibers. Morphological characteristics of nanofibers was evaluated using scanning electron microscopy, and formation of silver nanoparticles was confirmed performing field emission scanning electron microscopy and X-ray diffraction analysis. Mechanical and physical properties of the mats were studied to evaluate the effect of varous silver components. Antimicrobial properties of the fabricated scaffolds was examined with S.aureus. Capability cell seeding and biocompatibility of mats to human skin fibroblast and human bone marrow cells were investigated. The results indicate that the mats have proper mechanical properties with hydrophilic surface. The AgNps and chitosan- AgNPs diameter was in the range of 10-20 nm. it was shown that all selected silver components have equal and strong antimicrobial activity. However, mats loaded with chitosan-AgNps showed significantly more biocompatibility compare with other groups. Finally, due to the quality of nanofibers, antibacterial features and the ability of cellular attachments and proliferation the best silver components with optimum concentration was determined
  9. Keywords:
  10. Tissue Engineering ; Silver Nanoparticles ; Biomedical Applications ; Electrospinning ; Wound Dressing ; Biocompatibility

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