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Immersed sol-gel based amino-functionalized SPME fiber and HPLC combined with post-column photochemically induced fluorimetry derivatization and fluorescence detection of pyrethroid insecticides from water samples

Bagheri, H ; Sharif University of Technology | 2009

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  1. Type of Document: Article
  2. DOI: 10.1002/jssc.200900044
  3. Publisher: 2009
  4. Abstract:
  5. A method based on direct immersion solid-phase microextraction (DI-SPME) coupled with high performance liquid chromatography combined with post-column photochemically induced fluorimetry derivatization and fluorescence detection (HPLC-PIF-FD) was developed to extract three pyrethroid insecticides, i.e. cyfluthrin, cypermethrin, and flumethrin from water samples. A sol-gel based coating fiber using 3-(trimethoxysilyl propyl) amine as precursor was prepared and used for the extraction of the pyrethroids from groundwater samples. A post-column photochemical reactor was designed and constructed for the derivatization of these environmentally important pollutants to increase their fluorescence sensitivity and determination in HPLC. The parameters affecting extraction process (extraction time and temperature, pH, salt addition, and co-solvent) and desorption step (solvent, desorption time, and temperature) of the analytes from the sol-gel-based fiber, along with photochemical reaction conditions were investigated. The developed method proved to be relatively rapid, simple, and easy and offers high sensitivity and reproducibility. Linear dynamic ranges (LDR) for these insecticides were ranged between 0.25 to 50 μg/L. The regression coefficients were satisfactory (R2 > 0.984) for these pyrethroids. The limits of detection and limits of quantification varied between 0.09 and 0.35 μg/L and 0.25 and 1.00 μg/L, respectively. Relative standard deviation RSDs values varied between 4.41% and 6.20%. Relative recoveries obtained from analysis of Jajroud river water sample ranged between 94% and 104%. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA
  6. Keywords:
  7. High performance liquid chromatography-fluorescence detection ; Photochemically induced fluorimetry ; Pyrethroid insecticides ; Sol-gel-based solid phase microextraction ; Water samples ; Fluorimetry ; High performance liquid chromatography-fluorescence ; Pyrethroid insecticide ; Solid-phase microextraction ; Air pollution ; Amines ; Chromatographic analysis ; Column chromatography ; Desorption ; Esters ; Fluorescence ; Ground water ; High pressure liquid chromatography ; Hydrogeology ; Liquids ; Photochemical reactions ; Polymorphism ; Sol-gel process ; Sol-gels ; Sols ; Water analysis ; Solvent extraction ; Cipermethrin ; Cyfluthrin ; Flumethrin ; Insecticide ; Derivatization ; Fluorescence analysis ; High performance liquid chromatography ; Immersion ; pH ; Post column photochemical reactor ; Priority journal ; Process optimization ; Reactor ; Reproducibility ; River ; Sensitivity analysis ; Sol gel based solid phase microextraction ; Temperature ; Water pollutant ; Animals ; Chromatography, High Pressure Liquid ; Gels ; Humans ; Hydrogen-Ion Concentration ; Insecticides ; Molecular Structure ; Phase Transition ; Photochemical Processes ; Pyrethrins ; Reproducibility of Results ; Salts ; Sensitivity and Specificity ; Solvents ; Water Pollutants, Chemical ; Water Supply
  8. Source: Journal of Separation Science ; Volume 32, Issue 17 , 2009 , Pages 2912-2918 ; 16159306 (ISSN)
  9. URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.200900044