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Immobilization of Enzymes to Nanoparticles for use in Wastewater Treatment

Nisan, Roya Sadat | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50431 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran
  7. Abstract:
  8. Nowadays , application of enzymes as biocatalysts in wastewater treatment processes is increasing. It is economically important to reuse enzymes and increase their stability in these types of processes. Therefore, was immobilization of enzymes and using them in wastewater treatment have a great importance.In this project, Horseradish peroxidase enzyme, whose effect on phenol removal reactions in other studies has been proven before, was immobilized on the alginate-coated magnetic nanoparticles, to investigate the effect of immobilization on the stability and function of this enzyme for phenol removal. The enzyme was immobilized at the temperature of 25 ° C and pH = 8, according to the data that was optimized by the response surface method.In order to assure the effect of the immobilization on stability of HRP against time, temperature and pH changes, the immobilized and free enzyme were tested in different condition and the results shows the increase in stability against tempreture changes, pH variation and time Finaly the immobilized HRP was used for phenol removal and The analysis of the phenol removal shows that of 95% phenol contents was degraded at the minimum time of 145 minutes, with a hydrogen peroxide to phenol ratio of 1.2 and phenol concentrations of 10mM
  9. Keywords:
  10. Horseradish Peroxidase (HPR) ; Immobilization ; Magnetic Iron Oxide Nonoparticle ; Enzyme Stabilization ; Waste Treatment ; Phenol Removal

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