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Fabrication of in Situ Forming Bioadhesive Hydrogel for Cartilage Tissue Engineering

Abdolmaleki, Hamid | 2017

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50629 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Hasanzadeh, Zabihollah
  7. Abstract:
  8. In recent years, many efforts have been made in tissue engineering and new methods for the treatment of cartilage damage, with an emphasis on their non-invasive and less aggressive nature. Meanwhile, injectable and in situ forming hydrogels have been considered as a less invasive nature. On the other hand, lack of enough mechanical properties in these hydrogels is one of their main problem. In this study, gelatin and alginate was used to fabricate hydrogel as interpenetrating network (IPN) hydrogel and silica nano particles were also used to increase mechanical properties in the fabricating of hydrogels. Gelatin is also combined with dopamine in order to induce bio adhesive properties of hydrogel. The RSM method was used to design the experiments. using this method, two variables including gelation time and mechanical properties of hydrogel were optimized. HNMR and FTIR analysis were used to evaluate the synthesis of gelatin-dopamine conjugate and its crosslinking with glutaraldehyde. in order to analyze the surface morphology and porosity of hydrogel, SEM images were taken. MTT cell viability test were used to bio compatibility evaluation. Mechanical properties, gelation time, degradation rate, swelling ratio and adhesion to the tissue were analyzed on the hydrogel. The results showed that an injectable and in situ forming hydrogels with acceptable mechanical properties in cartilage tissue engineering is fabricated
  9. Keywords:
  10. Cartilage Tissue Engineering ; Interpenetrating Polymer Network ; Bioadhesive ; In-Situ Forming Hydrogel

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