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Fabrication of Scaffold with Microfluidic Channels for Heart Tissue Engineering

Momeni, Ehsan | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50628 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Saadatmand, Maryam
  7. Abstract:
  8. Myocardial infarction (MI) is one of the diseases caused by the temporary or permanent cramp of major coronary arteries. Due to this blockage, blood flow to the heart's myocardial tissue is greatly reduced and finally the person suffered from a Heart stroke (HS). Heart tissue engineering is a promising approach, based on the combination of cells and suitable biomaterials to develop and create heart-like biological substitutes. Since high cardiac cell density, providing metabolic needs like oxygen and nutrients was a challenge. So creation of blood vessel networks within this type of designed tissue has been considered very much.The purpose of this project is to construct scaffolds with microfluidic channels in order to oxygenate better to cells in depth of scaffolds. Molds for the fabrication of these scaffolds were made. In this study, Alginate and gelatin polymers were used to construct scaffolds including interconnected polymeric networks. FTIR results showed crosslinking between gelatin functional groups by glutaraldehyde. The scaffold containing 1% alginate and 2.5% gelatin based on Young's modulus, had the most similarity to the natural tissue of the heart and considered as an optimum scaffold.FESEM images showed that the optimum scaffold had pore size, interconnectivity of pores and porosity needed for growth and viability of cells. The scaffolds showed suitable degradation rate and swelling rate for cell adhesion and cell growth. In order to evaluate cell viability, human lung cancer epithelial cell line (A549) encapsulated inside the scaffolds and MTT test was performed 24 and 72 hours after. Cell viability results showed that both increasing the number of micro-channels in the scaffold and dynamic culture conditions, in comparison to static culture improves the viability of the encapsulated cells. In addition, by comparing the viability results of day 1 and 3, it was concluded that cells proliferated
  9. Keywords:
  10. Heart Tissue Engineering ; Hydrogel Scaffold ; Bioreactor ; Encapsulation ; Microfluidic System ; Microfluidic Channels ; Dynamic Cell Culture

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