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Device on a Chip, in Order to Control and Separation of Circulating Tumor Cells

Masoudi, Mohammad Mahdi | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50791 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir; Khodaygan, Saeed
  7. Abstract:
  8. The process of separating cells from a laboratory sample, is a crucial step in biotechnology and medical science. Cells separation is important for various reasons, including the identification and treatment of diseases, as an example, separation of Circulating Tumor Cells (CTCs) from patient blood sample. CTCs are cells that shed from a tumor and diffuse into blood vessels. These cells are the starters of metastasis, the process in which cancer spreads in body, and are main reason of deaths caused by cancer. Extremely low percentages of target cells in the blood, clarify the importance of highly accurate and sensitive separation. Currently, conventional separation methods are of macro-order and requiring large sample, therefore, are very expensive. Microfluidics, the science of syudying fluid flow in very small volumes, can be used as a efficient, portable, accurate and inexpensive alternative to current methods. In this study, using inertial michrofluidics, separation of CTCs is performed based on their different size relative to other cells. Subsequently, by synthesizing magnetic nanoparticles and attaching them to antibodies, it is possible to accurately capture a single CTC
  9. Keywords:
  10. Tumors ; Cancer ; Cell Separation ; Microfluidic Chip ; Magnetic Nanodot ; Circulating Tumor Cells (CTC) ; Intellectualized Magnetic

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