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Hybrid System for Growth Factor Delivery

Torabi Rahvar, Parisa | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51056 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Abdekhodaie, Mohammad Jafar
  7. Abstract:
  8. Platelet Rich Plasma (PRP) is a blood-derived product containing concentrate of platelets, which are a rich source of autologous growth factors. PRP injection has been used clinically as a therapeutic method for cartilage repair. However, clinical efficiency of this method is unpredictable, maybe as a result of burst release of growth factors then fail cell-stimulating potential as most biomolecules are cleaned before they can exert a therapeutic effect. The aim of this project was to prepare a suitable scaffold for PRP delivery to regenerate cartilage injuries. In order to resemble polysaccharide-protein nature of the cartilaginous extracellular matrix, in this study, we developed an injectable enzymatically crosslinked hydrogels composed of type II collagen-tyramine and hyaluronic acid-tyramine conjugates which form hydrogel in the presence of horseradish peroxidase (HRP) and non-toxic concentration of hydrogen peroxide (H2O2). Type II collagen was extracted from chicken sternum. The properties of hybrid hydrogels were characterized in terms of gelation time, swelling ratio, morphology and rheological characterization. To evaluate the efficiency of this scaffold as the PRP delivery system, release of a model protein, BSA, was studied. The results showed that the hybrid system is more efficient for protein delivery than the mono-component scaffold. It is expected that the novel type II collagen/hyaluronic acid scaffold presented here, to be useful biomaterial scaffold for cartilage regeneration
  9. Keywords:
  10. Hybrid System ; Injectable Hydrogel ; Cartilage Tissue Engineering ; Platelet Rich Plasma (PRP) ; Protein Delivery ; Collagen

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