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Covalent immobilization of cellulase using magnetic poly(ionic liquid) support: improvement of the enzyme activity and stability

Hosseini, H ; Sharif University of Technology | 2018

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  1. Type of Document: Article
  2. DOI: 10.1021/acs.jafc.7b03922
  3. Publisher: American Chemical Society , 2018
  4. Abstract:
  5. A magnetic nanocomposite was prepared by entrapment of Fe3O4 nanoparticles into the cross-linked ionic liquid/epoxy type polymer. The resulting support was used for covalent immobilization of cellulase through the reaction with epoxy groups. The ionic surface of the support improved the adsorption of enzyme, and a large amount of enzyme (106.1 mg/g) was loaded onto the support surface. The effect of the presence of ionic monomer and covalent binding of enzyme was also investigated. The structure of support was characterized by various instruments such as FT-IR, TGA, VSM, XRD, TEM, SEM, and DLS. The activity and stability of immobilized cellulase were investigated in the prepared support. The results showed that the ionic surface and covalent binding of enzyme onto the support improved the activity, thermal stability, and reusability of cellulase compared to free cellulase. © 2018 American Chemical Society
  6. Keywords:
  7. Bins ; Crosslinking ; Enzyme immobilization ; Enzymes ; Ionic liquids ; Iron compounds ; Liquids ; Nanomagnetics ; Reusability ; Covalent binding ; Covalent immobilization ; Fe3O4 nanoparticles ; Magnetic nanocomposites ; Magnetic support ; Poly(ionic liquid)s ; Support surfaces ; Enzyme activity ; Ferric ion ; Immobilized enzyme ; Ionic liquid ; Magnetite nanoparticle ; Polymer ; Chemistry ; Heat ; Magnetism ; Metabolism ; Adsorption ; Cellulase ; Enzyme Stability ; Enzymes, Immobilized ; Ferric Compounds ; Hot Temperature ; Magnetic Phenomena ; Magnetite Nanoparticles ; Polymers
  8. Source: Journal of Agricultural and Food Chemistry ; Volume 66, Issue 4 , 2018 , Pages 789-798 ; 00218561 (ISSN)
  9. URL: https://pubs.acs.org/doi/10.1021/acs.jafc.7b03922