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Design of Chemiluminescence Sensors Enhanced by Colloidal Nanoparticles for Discrimination of Biomolecules

Shahrajabian, Maryam | 2018

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  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 51565 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Hormozinezhad, Mohammad Reza
  7. Abstract:
  8. In the first part of this research, we introduce a novel chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as “fingerprints” and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between biothiols in a wide concentration range( i.e. 25-100 µM for cysteine, 5-800 µM for both glutathione and glutathione disulfide). Furthermore, the proposed sensor array could efficiently discriminate the individual thiols and their mixture. Moreover, it was applied to distinguish among the above analytes in human plasma.
    In the second part of this research, we have developed a chemiluminescence based sensor array for discriminating a set of NPs. The proposed method is based on the enhancement effect of NPs on Luminol–oxidant CL intensity by their catalytic effect. Three kinds of oxidant including H2O2, AgNO3, and K3Fe(CN)6 were used as sensor elements and NPs exhibited diverse enhancing responses to different oxidant-Luminol CL systems producing unique response patterns that were identified through heat map and chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). Five NPs have been well distinguished at various concentrations and from their mixtures. In addition, this method clearly revealed a linear relationship between CL signal values and the concentrations of NPs for the quantitative detection of NPs.
    In the third part of this research, we have reported a simple strategy for chiral recognition of thiol-containing amino acids including penicillamine (PA) and cysteine (Cys). We have successfully designed a nanoparticle-based chemiluminescence (CL) system based on the reaction between cadmium telluride quantum dots (CdTe QDs) and enantiomers. The different interactions of CdTe QDs with PA enantiomers or Cys enantiomers led to different CL intensities, resulting in the chiral recognition of these enantiomers. The developed method showed the ability for determination of enantiomeric excess of PA and Cys. To demonstrate potential applications of this method, the designed platform was applied for the quantification of PA in urine and tablet samples
  9. Keywords:
  10. Catalytic Effect ; Nanoparticles ; Sensors Array ; Biomolcule Recognition ; Penicillamine ; Enantiomers

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