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Studying Graphene Oxide Nanoflakes Size Effect on Antibiotic Resistant Bacteria

Homa, Kinza | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: English
  3. Document No: 52233 (04)
  4. University: Sharif University of Technology
  5. Department: Physics
  6. Advisor(s): Naseri, Naimeh
  7. Abstract:
  8. Two-dimensional (2D) nanomaterials are ultrathin nanomaterials with a high degree of anisotropy and chemical functionality. Research on 2D nanomaterials is still in its infancy, with the majority of research focusing on elucidating unique material characteristics and few reports focusing on biomedical applications of 2D nanomaterials. Here we report the properties and application of 2Dnanomaterials in field of bio technology while used main material in our project is graphene oxide for investigating its antibacterial properties. Because the properties of graphene oxide (GO)-based materials strongly depend on the lateral size and size distribution of GO nanosheets; therefore, GO and its derivatives with narrow size distributions are highly desired. Specifically, graphene oxide sheets show good chemical, physical and antibacterial properties and it is gaining attention of certain researchers because of its considerably less toxicity inside the eukaryotic cells. This remarkable property has driven the trend to its use in many biological applications. In our project, the effect of modified graphene oxide plates on three bacteria of Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter Baumannii was investigated. Also, using sonication and centrifuge physical methods, graphene oxide was prepared with different sheet sizes. We prepared different flake sizes from around 72 μm2 to 0.004 μm2. Size analysis was done by AFM. The structural and optical properties of the as-prepared GO nanosheets were characterized by XRD, Raman, FTIR, and UV-vis microscopy. MIC tests were performed, and counting bacteria over time with graphene oxide interaction has also been done. The interaction between GO and bacteria is studied by FESEM. According to our results the growth of Staphylococcus aureus bacteria is stopped by graphene oxide when its size is around 0.063 μm2 and when its area decreases for about 0.018 μm2 the effect is shown for Pseudomonas aeruginosa bacteria. These results indicate that the GO nanosheets could be used as a promising new material for antibacterial activity
  9. Keywords:
  10. Graphene Oxide ; Graphene Nanosheets ; Antibacterials ; Antibacterial Resistance ; Nanoflakes

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