Loading...

Synthesis and Preparation of Nanostructured Ag@SiO2 Core Shells with Different Morphologies and their Antibacterial Properties

Malekzadeh, Mahdieh | 2019

519 Viewed
  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 52365 (07)
  4. University: Sharif University of Technology
  5. Department: Materials Science and Engineering
  6. Advisor(s): Halali, Mohammad
  7. Abstract:
  8. In this study, silver nanoparticles (Ag NPs) with different morphologies were synthesize by physical and chemical methods. Spherical silver nanoparticles were synthesized by electro magnetic levitation melting approach, while silver nanoplates and nanorods were prepared by chemical reduction route. Ag NPs were then coated with thin porous silica shell. The dissolution behavior of synthesized Ag@SiO2 was investigated in PBS at 25, 37 and 55 °C. Spherical and triangular Ag@SiO2 were conjugated by Penicillin G molecules. The silver and penicillin contents of the synthesized nano-antibiotics were determined by ICP-OES and TGA analyses. The nanoparticles were characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The antibacterial properties and synergistic effects of nanostructured Ag@SiO2 and Ag@SiO2–Penicillin on killing the Methicillin-susceptible S. aureus (MSSA) and Methicillin-resistant S. aureus (MRSA) bacteria were also examined. The Fractional inhibitory concentration (FIC) indexes showed the synergistic antibacterial properties of Ag@SiO2–Penicillin. The minimum inhibitory concentrations (MICs) of spherical and triangular Ag@SiO2–Penicillin were 155 and 130 µg/ml against MRSA, respectively. Grafting of about 2.6 wt% penicillin on Ag@SiO2 remarkably enhanced bactericidal properties against MSSA and MRSA bacteria and showed no cell-death inducer effects towards A431 cell line
  9. Keywords:
  10. Silver Nanoparticles ; Staphylococcus Aureus ; Penicillin ; Antibacterials ; Synergistic Effect ; Core-Shell Nanostructure

 Digital Object List

 Bookmark

No TOC