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Developing Protein-Loaded Modified Hyaluronic Acid Hydrogel for Tissue Regeneration

Asadi Korayem, Maryam | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52725 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Abdekhodaie, Mohammad Jafar
  7. Abstract:
  8. Growth factors play an important role in tissue repair and regeneration including articular cartilage tissue. Direct injection of growth factor is a common practice in clinics. However, it has been shown that this method is not very effective and, in some cases, induces some side effects. Moreover, incorporation of this valuable signals in systems where they are quickly released and washed out is not effective as well. Thereby the aim of this study was to design and fabricate an efficient system to effectively deliver growth factors for cartilage tissue engineering. The designed system is an injectable, in situ forming hydrogel, based on hyaluronic acid (HA) as a biocompatible and attractive polymer in cartilage tissue engineering. The crosslinking method used in this study is the enzymatic method based on HRP and hydrogen peroxide. To induce growth factor binding, sulfate groups are conjugated to the HA. This idea is taken from heparin, as a highly sulfated natural polymer with high growth factor binding. The injectable enzymatically crosslinked hydrogel based on sulfated HA is fabricated in this study for the first time. It is shown that sulfate groups increase hydrogel swelling and decrease its enzymatic degradation rate. Moreover, the sulfated HA significantly improves TGF-β1 sequestration, thereby effectively extending the availability of the growth factor in the hydrogel. Furthermore, the sulfate modification not only does not impair cell compatibility but although increases its elongation, attachment and its metabolic activity. Moreover, differentiation studies showed that the incorporation of sulfate groups promotes the chondrogenesis and ECM production of encapsulated chondroprogenitor cells
  9. Keywords:
  10. Articular Cartilage ; Tissue Engineering ; Injectable Hydrogel ; Growth Factor ; Horseradish Peroxidase (HPR) ; Hyaluronic Acid Gel ; Chondrogenesis Process

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