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Graphene oxide-l-arginine nanogel: A pH-sensitive fluorouracil nanocarrier

Malekimusavi, H ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1002/bab.1768
  3. Publisher: Wiley-Blackwell Publishing Ltd , 2019
  4. Abstract:
  5. Nowadays, putting forward an accurate cancer therapy method with minimal side effects is an important topic of research. Nanostructures, for their ability in controlled and targeted drug release on specific cells, are critical materials in this field. In this study, a pH-sensitive graphene oxide-l-arginine nanogel was synthesized to carry and release 5-fluorouracil. Optimized conditions using statistical analysis, based on the maximum relative viscosity of nanogel, were evaluated: 5.489 for the concentration of l-arginine and 2.404 for pH. The prepared nanogels were characterized using scanning electron microscope and transmission electron microscope images and Fourier-transform infrared spectroscopic analysis. Cytotoxicity was assessed using the sulforhodamine B (SRB) assay on MCF-7 breast cancer cells. The fluorouracil release was measured by the dialysis bag method, UV spectrophotometry, and fluorouracil calibration diagram. Results proved the successful controlled release of fluorouracil at pH 5.4 and the beneficial role of graphene-oxide- l-arginine- fluorouracil nanogel in eliminating cancer cells. © 2019 International Union of Biochemistry and Molecular Biology, Inc
  6. Keywords:
  7. Graphene-oxide ; Amino acids ; Arginine ; Cells ; Diseases ; Drug delivery ; Graphene ; Graphene oxide ; Nanostructured materials ; PH sensors ; Positive ions ; Scanning electron microscopy ; Spectroscopic analysis ; Targeted drug delivery ; Transmission electron microscopy ; Fluorouracil ; Fourier transform infra reds ; L-Arginine ; MCF-7 breast cancer cells ; Nanogels ; Optimized conditions ; Transmission electron ; UV spectrophotometry ; Controlled drug delivery ; Arginine ; Sulforhodamine B ; Arginine ; Macrogol ; NanoGel ; Nanoparticle ; Angiogenesis ; Apoptosis ; Article ; Breast cancer ; Controlled drug release ; Cytotoxicity ; Fourier transform infrared spectroscopy ; Gel ; MCF-7 cell line ; PH ; Ultraviolet spectrophotometry ; Cell survival ; Chemistry ; Drug effect ; Human ; Surface property ; Arginine ; Cell Survival ; Graphite ; Humans ; Hydrogen-Ion Concentration ; MCF-7 Cells ; Nanoparticles ; Particle Size ; Polyethylene Glycols ; Polyethyleneimine ; Surface Properties
  8. Source: Biotechnology and Applied Biochemistry ; Volume 66, Issue 5 , 2019 , Pages 772-780 ; 08854513 (ISSN)
  9. URL: https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/bab.1768