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Investigation and Determination of Optimal Composition of Hydrogels and Nanoparticles for Proliferation of Mesenchymal Stem Cells in Alginate Beads

Akbari Kenari, Mahsa | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 53475 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Yaghmaei, Soheila; Arpanaei, Ayyoob
  7. Abstract:
  8. The aim of this dissertation is to investigate and determine the optimal composition of hydrogels that can meet the needs of stem cells for the growth and proliferation of mesenchymal stem cells. In this research, firstly, unfunctionalized and functionalized mesoporous silica nanoparticles (with an average diameter of approximately 90 nm), were synthesized. Scanning electron microscopy and zeta potential measurement were utilized to study their morphology and surface charge. Then, graphene oxide nanosheets were synthesized. Field emission electron microscopy, zeta potential measurement and Fourier transform infrared spectrum analysis were used to examine their morphology, surface charge and their functional groups. Then, alginate beads containing gelatin with 2% CaCl2, 1% CaCl2 and 1% SrCl2.6H2O, 2% SrCl2.6H2O as crosslinkers were prepared in the absence of cells and their sizes were 1.49, 1.82 and 2.03 mm, respectively. The toxicity of aminated silica mesoporous nanoparticles and graphene oxide nanosheets with different concentrations on mesenchymal stem cells in 2D culture medium was investigated. In the next step, the proliferation and viability of encapsulated cells in alginate beeds containing gelatin with three types of crosslinkers mentioned on days 0, 1, 3, 7 and 14 were evaluated. The results showed that the combination of aminated silica mesoporous nanoparticles and graphene oxide nanosheets increased the viability of mesenchymal stem cells in 2D culture medium. In addition, the stability of alginate hydrogels with gelatin and the proliferation and survival of encapsulated mesenchymal stem cells within the hydrogel are increased by the addition of strontium ions (in low concentration (1% w/v)) as a crosslinker along with calcium. The results of this study show that these and similar systems can be used for proliferation and differentiation of stem cells for the treatment of diseases in the field of personalized medicine
  9. Keywords:
  10. Mesoporous Silica Nanoparticles ; Magnetic Graphene Oxide Nano-Sheets ; Bone Marrow Mesenchymal Stem Cells ; Three Dimensional Scaffolds ; Metal Ions ; Strontium Metal Ion ; Alginate/Gelatin Beads

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