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Synthesis, characterization and mechanistic study of nano chitosan tetrazole as a novel and promising platform for CRISPR delivery

Rabiee, N ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1080/00914037.2020.1809405
  3. Publisher: Taylor and Francis Inc , 2020
  4. Abstract:
  5. Here, a newly modified chitosan tetrazole was synthesized and fully characterized for CRISPR technology since by increasing the ratio of nitrogen to other elements, the interaction between the genetic material and this nanomaterial was significantly enhanced. The nanomaterial was added to HEK-293 cells for pCRISPR delivery. The nanomaterials were optimized and showed an acceptable binding ability with DNA due to its increasing zeta potential in the synthesized chitosan tetrazole in comparison with chitosan, as well as considerable transfection efficiency, more than 25%, and, thus, can be considered as a promising and also new gold-standard for the delivery of pCRISPR. © 2020 Taylor & Francis Group, LLC
  6. Keywords:
  7. Chitosan tetrazole ; Chitosan-based non-viral vector ; Gene delivery ; PCRISPR ; Nanostructured materials ; Binding abilities ; Genetic materials ; Gold standards ; HEK293 cells ; Mechanistic studies ; Modified chitosan ; Tetrazoles ; Transfection efficiency ; Chitosan
  8. Source: International Journal of Polymeric Materials and Polymeric Biomaterials ; 2020
  9. URL: https://www.tandfonline.com/doi/abs/10.1080/00914037.2020.1809405?journalCode=gpom20