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Immobilization of laccase from trametes hirsuta onto CMC coated magnetic nanoparticles

Sadeghzadeh, S ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.5829/IJE.2020.33.04A.01
  3. Publisher: Materials and Energy Research Center , 2020
  4. Abstract:
  5. In this study Fe3O4/CMC magnetic nanoparticles were synthesized through co-precipitation method. Afterward, laccase from Trametes hirsuta was immobilized onto Carboxymethyl cellulose (CMC)-coated magnetic Fe3O4 nanoparticles by covalent bonding between carboxyl groups of carboxymethyl cellulose and amine group of laccases. Also, the resulted magnetic nanoparticles and immobilized laccase were characterized by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and dynamic light scattering (DLS) analysis. Moreover, the vital factors in enzyme immobilization, such as contact time, amount of N-hydroxysuccinimide (NHS), and the amount of nanoparticles were optimized, which successively 48 h, 0.01 g, and 0.0125 g were achieved for 0.01g of N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide (EDC). Activity recovery of 51 ± 0.8% was achieved by optimizing the immobilization process. The results also indicated that the loading of laccase onto carboxymethyl cellulose-coated Fe3O4 nanoparticles was approximately 120 (mg/g). Finally, the immobilized laccases on magnetic support could save nearly 50% of their initial activity after five consecutive cycles. © 2020 Materials and Energy Research Center. All rights reserved
  6. Keywords:
  7. Fe3O4/cmc ; Immobilization ; Trametes hirsute ; Cellulose ; Dynamic light scattering ; Enzyme immobilization ; Fourier transform infrared spectroscopy ; Iron oxides ; Magnetism ; Magnetite ; Precipitation (chemical) ; Scanning electron microscopy ; Synthesis (chemical) ; Activity recovery ; Carboxy-methyl cellulose ; Coprecipitation method ; Fe3O4 nanoparticles ; Immobilization process ; Immobilized laccase ; Magnetic support ; N-hydroxysuccinimide ; Magnetic nanoparticles ; Carboxymethyl Cellulose ; Laccase ; Magnetic Properties
  8. Source: International Journal of Engineering, Transactions A: Basics ; Volume 33, Issue 4 , 2020 , Pages 513-519
  9. URL: http://www.ije.ir/article_106022.html