Design a Microfluidics System for Drug Testing of Leukemia Patient's Bone Marrow Aspirate

Rahimi, Ali | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 53662 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Saidi, Mohammad Said
  7. Abstract:
  8. Cancer is one of the most causes of mortality in the world. Leukemia is a type of cancer initiated by the rapid proliferation of blood cells including WBCs, RBCs, and Platelets, that classified into four general types of AML, ALL, CML, and CLL. The main treatment of leukemia is chemotherapy. Because of many problems that patients have with this treatment and not so good results of chemotherapy, we design a microfluidic chip that can perform personalized medicine treatment for leukemia patients.This microchip consists of two main parts, Concentration Gradient Generator (CGG), and cell culture. CGG system is a tree CGG with two inlets and four outlets, which makes a linear concentration of drug in outlets with mixing efficiency above 98 percent. In cell culture part, AML cells and mesenchymal stem cells (MSCs) are cultured in cell chambers and in a co-culture system. MSCs in the bottom of cell chamber and AML cells are cultured suspended. The cells are obtained from bone marrow aspirate.Geometry of chip optimized based on parameters that affects cells, like oxygen and glucose concentration and shear stress in cell chamber. Microchip consists of three drug testing systems that enable parallel tests by three drug on three different cells or three different times
  9. Keywords:
  10. Lab-on-a-Chip ; Cell Culture ; Concentration Gradient Generator ; Leukemia ; Microfluidic System ; Personalized Medicine

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