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Design and Fabrication of a Microfluidic System for Cell Counting and Sizing

Ahmadi, Hesameddin | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 53699 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Taghipoor, Mojtaba; Hosseini, Vahid
  7. Abstract:
  8. The growing demand for diagnostic tools with enhanced analytical characteristics in terms of sensitivity, selectivity, and low response time has encouraged researchers to conduct their research towards the development of point-of-care (POC) biosensors. POC diagnostic devices are powerful tools for the detection, diagnosis, and monitoring of diseases at their initial stages. Microfluidic cytometers based on the Coulter principle have recently shown a great potential for POC biosensors for medical diagnosis. One of the most important components in detecting cells by a Coulter counter is a micropore or sensing gate. Therefore, in this study, for counting and sizing the cells using the resistive pulse sensing (RPS) method, first, new and cost-effective methods for making micropores with a low aspect ratio and suitable for sensing cells were investigated, and finally, the zero-length method using cutter blade selected. After that, a system for making micropore was designed and fabricated based on molding from the intersection point of two blades, and over time, its problems were identified and resolved. Then, to evaluate the performance of the micropore fabrication system and to determine the characteristics of the micropores obtained from this system, the micropores were examined electrochemically and also by a microscope. After making a suitable micropore, the microfluidic and electrical system required for cell counting and sizing was designed and built. Yeast cells were then used as a model organism to evaluate the function of the cell counting and sizing system. After that, the results obtained from the yeast cell test were analyzed by MATLAB software, and finally, using the results obtained from the RPS test and the results obtained from conventional cell counting and sizing methods, the cell counting and sizing system was calibrated
  9. Keywords:
  10. Lab-on-a-Chip ; Cell Size ; Microfluidic System ; Resistive Pulse Sensing ; Cytometry ; Cell Counting ; Micropore Fabrication

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