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A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR

Jalili, A ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.1038/s41598-021-02535-1
  3. Publisher: Nature Research , 2021
  4. Abstract:
  5. Polymerase chain reaction (PCR) is a powerful tool for nucleic acid amplification and quantification. However, long thermocycling time is a major limitation of the commercial PCR devices in the point-of-care (POC). Herein, we have developed a rapid droplet-based photonic PCR (dpPCR) system, including a gold (Au) nanofilm-based microfluidic chip and a plasmonic photothermal cycler. The chip is fabricated by adding mineral oil to uncured polydimethylsiloxane (PDMS) to suppress droplet evaporation in PDMS microfluidic chips during PCR thermocycling. A PDMS to gold bonding technique using a double-sided adhesive tape is applied to enhance the bonding strength between the oil-added PDMS and the gold nanofilm. Moreover, the gold nanofilm excited by two light-emitting diodes (LEDs) from the top and bottom sides of the chip provides fast heating of the PCR sample to 230 °C within 100 s. Such a design enables 30 thermal cycles from 60 to 95 °C within 13 min with the average heating and cooling rates of 7.37 ± 0.27 °C/s and 1.91 ± 0.03 °C/s, respectively. The experimental results demonstrate successful PCR amplification of the alcohol oxidase (AOX) gene using the rapid plasmonic photothermal cycler and exhibit the great performance of the microfluidic chip for droplet-based PCR. © 2021, The Author(s)
  6. Keywords:
  7. Polymerase chain reaction (PCR) ; Point-of-care (POC) ; Droplet-based photonic PCR (dpPCR) system ; Polydimethylsiloxane ; Microfluidic chips ; Light-emitting diodes (LEDs) ; Alcohol oxidase (AOX)
  8. Source: Scientific Reports ; Volume 11, Issue 1 , December , 2021 ; 20452322 (ISSN)
  9. URL: https://www.nature.com/articles/s41598-021-02535-1