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Improving the Biomedical Performance of Metal–Organic Frameworks in Drug Delivery Systems: in Vitro Studies

Bahmanpour, Maryam | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 55397 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Bagherzadeh, Mojtaba
  7. Abstract:
  8. In this study, water-stable metal-organic frameworks, UiO-66-(CO2H)2, MIL-53-NH2 (Al), ZIF-8 and MIP-202 (Zr), were selected to study the influence of using plant extracts in drug loading process and also its targeted release from these compounds. For this purpose, at first the solution of different plant extracts was used separately as a synthetic solvent in the preparation of the desired MOFs to be present in the structure of these compounds and increase the interaction of the drug with the nanocarrier. Then, a coating of plant extract was applied on the nanocarrier containing the drug after loading the drug to prevent the rapid penetration of the drug from the cavities of MOFs (drug withdrawal from the cavity) and also the penetration of phosphate into metal clusters on the surface of some MOFs (nanocarrier destruction) and prevented the burst release of the drug. Therefore, after characterizing of the synthesized nanocarriers by different analyses methods, the loading rate of doxorubicin in them was calculated and its release profile at different pHs was investigated. Cytotoxicity and entry of nanocarriers into the target cell are also studied. The results of drug loading showed that doxorubicin was loaded on nanocarriers MIP-202/TE, ZIF-8/TPE, UiO-66-(CO2H)2/TPE, and MIL-53-NH2/BEe by 5.49%, 0.61%, 2.58%, and 3.64%, respectively. In addition, the relative cell viability of the synthesized nanomaterials is above 80%, which decreases to 42% after DOX loading and rises to above 79% again due to being covered with plant extract. The results show that the use of a very cheap and green coating of the plant extract, while significantly increasing the amount of drug loaded and providing controlled and pH-sensitive release, also reduces the toxicity of the resulting nanocarrier and delivers the drug more effectively to the target cell.
  9. Keywords:
  10. Doxorubicin ; Drug Delivery ; In-Vitro Biological Assays ; Metal-Organic Framework ; PH-triggered Release ; Natural Extract ; Controlled Release

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