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Engineered hyaluronic acid-decorated niosomal nanoparticles for controlled and targeted delivery of epirubicin to treat breast cancer

Mansoori Kermani, A ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1016/j.mtbio.2022.100349
  3. Publisher: Elsevier B.V , 2022
  4. Abstract:
  5. Targeted drug delivery systems using nanocarriers offer a versatile platform for breast cancer treatment; however, a robust, CD44-targeted niosomal formulation has not been developed and deeply studied (both in vitro and in vivo) yet. Here, an optimized system of epirubicin (Epi)-loaded niosomal nanoparticles (Nio) coated with hyaluronic acid (HA) has been engineered for targeting breast cancer cells. The nanoformulation was first optimized (based on size, polydispersity index, and entrapment efficiency); then, we characterized the morphology, stability, and release behavior of the nanoparticles. Epirubicin release from the HA-coated system (Epi-Nio-HA) showed a 21% (acidic buffer) and 20% (neutral buffer) reduction in comparison with the non-coated group (Epi-Nio). The cytotoxicity and apoptosis results of 4T1 and SkBr3 cells showed an approximately 2-fold increase in the Epi-Nio-HA system over Epi-Nio and free epirubicin, which confirms the superiority of the engineered nanocarriers. Moreover, real-time PCR data demonstrated the down-regulation of the MMP-2, MMP-9, cyclin D, and cyclin E genes expression while caspase-3 and caspase-9 gene expression were up-regulated. Confocal microscopy and flow cytometry studies uncovered the cellular uptake mechanism of the Epi-Nio-HA system, which was CD44-mediated. Furthermore, in vivo studies indicated Epi-Nio-HA decreased mice breast tumor volume by 28% (compared to epirubicin) without side effects on the liver and kidney. Conclusively, our results indicated that the HA-functionalized niosomes provide a promising nanoplatform for efficient and targeted delivery of epirubicin to potentially treat breast cancer. © 2022 The Authors
  6. Keywords:
  7. Active targeting ; Breast cancer ; CD44 ; Controlled drug delivery ; Hyaluronic acid ; Nanomedicine ; Cancer cells ; Cell death ; Diseases ; Gene expression ; Mammals ; Nanoparticles ; Organic acids ; Polymerase chain reaction ; Targeted drug delivery ; Acid systems ; Controlled-delivery ; Epirubicin ; Genes expression ; Nanocarriers ; Targeted delivery ; Targeted drug delivery systems ; Medical nanotechnology
  8. Source: Materials Today Bio ; Volume 16 , 2022 ; 25900064 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S2590006422001478