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Poly-L-lysine/hyaluronan nanocarriers as a novel nanosystem for gene delivery

Souri, M ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1111/jmi.13107
  3. Publisher: John Wiley and Sons Inc , 2022
  4. Abstract:
  5. The present research comes up with a novel DNA-loaded poly-L-lysine (PLL)/hyaluronan (HA) nanocarrier (DNA-loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA-loaded PLL molecules by HA. Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission-scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) were used to analyse the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of –24 ± 3 mV, with an average size of 138 ± 6 nm, in an ellipsoid-shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non-toxic to the cells. Furthermore, the uptake of the DNA-loaded PLL/HA NCs by the human embryonic kidney (HEK)-293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK-293T cells at the optimised conditions. Therefore, the DNA-loaded PLL/HA NCs are the potent strategy for developing nanosystems for gene delivery applications. © 2022 Royal Microscopical Society
  6. Keywords:
  7. Gene delivery ; Hyaluronan ; Nanocarriers ; Poly-L-lysine ; DNA ; Nanocarrier ; Biocompatibility ; Controlled study ; Cytotoxicity ; Field emission scanning electron microscopy ; Flow cytometry ; Fourier transform infrared spectroscopy ; Gene delivery system ; Gene expression profiling ; HEK293T cell line ; Human ; Human cell ; In vitro study ; MTT assay ; Particle size ; Photon correlation spectroscopy ; Physical chemistry ; Transmission electron microscopy ; Chemistry ; Genetics ; Infrared spectroscopy ; Humans ; Hyaluronic Acid ; Microscopy, Electron, Transmission ; Polylysine ; Spectroscopy, Fourier Transform Infrared
  8. Source: Journal of Microscopy ; Volume 287, Issue 1 , 2022 , Pages 32-44 ; 00222720 (ISSN)
  9. URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/jmi.13107