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An Investigation on the Parameters Affecting Electrochemical Biosensors Performance to Detect Prostate Cancer

Saghafi, Mohammad Javad | 2022

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 56050 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Abdekhodaie, Mohammad Jafar
  7. Abstract:
  8. Early detection of prostate cancer using biosensors has been a subject of research for nearly two decades due to its critical importance. The timing of detection plays a crucial role in determining treatment success rates. However, a significant limitation of these studies has been the cost and limited sensitivity of the sensors. In this research, various treatments used in biosensors were compared and necessary parameters to develop a sensitive, low-cost sensor have been subject of optimization. For this reason, we used pencil graphite and graphite sheet electrodes, both of which have potential for low-cost production on a large scale. Additionally, we tested graphene oxide, amine-treated graphene oxide, amine-treated carbon black, and reduced graphene oxide nanoparticles for surface modification. Antibody immobilization was achieved through two different methods and compared. The results confirmed successful immobilization of antibodies and identification of different concentrations of prostate-specific antigen. Furthermore, it is found that graphite sheets as a base material and EDC/NHS as a modifier were more suitable options for developing this electrochemical biosensor. Although our focus was on prostate cancer, the scope of this research is broader. During our comparison of pencil graphite and graphite sheet electrodes, we observed a seventeen-fold increase in faradic current flux, leading us to conclude that graphite sheets are more suitable for this application. Additionally, nitric acid treatment and carbon black nanoparticles increased current by fifteen and two times, respectively. Lastly, carboxyl group activation proved to be a better method for antibody immobilization than amine-glutaraldehyde crosslinking, as it can immobilize nearly three times more antibodies on a given surface
  9. Keywords:
  10. Early Detection ; Cancer Diagnosis ; Prostate Cancer ; Electrochemical Biosensors ; Graphene Oxide

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