Design, Simulation, and Fabrication of DEP-Based Microfluidic Chip for Bioparticles Separation

Vamegh, Reza | 2023

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 56294 (05)
  4. University: Sharif University of Technology
  5. Department: Electrical Engineering
  6. Advisor(s): Fardmanesh, Mehdi
  7. Abstract:
  8. Microfluidics, employing fluids in structures with micrometer dimensions, is an emerging technology that has brought great opportunities to develop various devices including lab-on-a-chip systems. Lab-on-a-chip systems have the advantages of integrating several stages of different analytical methods, application in diverse fields, consumption of small volumes of samples, and portability. One of the crucial applications of lab-on-a-chip devices is sorting cells and biological particles, done by different methods. In separator devices, high separation efficiency and repeatability are critical. When a neutral particle is polarized in a non-uniform electric field, the dielectrophoresis force acts on it. In recent years, the integration of dielectrophoresis as an efficient, fast, and accurate separation technique with microfluidic systems has attracted wide attention for many applications, including separating particles with micron and nano dimensions. Separation based on dielectrophoresis is performed in two general categories: separation based on the difference in particle size and separation based on the distinct structural properties of the particles. Based on the dominant effect of the size of the particles on the dielectrophoresis force compared to their structural difference, separation based on the structural difference of particles with similar dimensions is more challenging and requires more delicacy in design. In this study, we design, simulate, fabricate, and evaluate the performance of two distinct microfluidic chips for the separation of live and dead yeast cells, with a diameter of 6 and 5 microns, respectively, based on the phenomenon of dielectrophoresis. In this regard, we will first review the principles of microfluidics, the history of microfluidic devices and their applications, cell separation techniques as a bold application, the theory governing dielectrophoresis, and the parameters affecting it. In the following, we devote a chapter to the simulations performed to design and optimize the separator structures. Then the different stages of fabrication and some challenges in the practical implementation of the separator chips are explained. Finally, the results obtained by testing the chips are presented
  9. Keywords:
  10. Polarization ; Separation ; Bioparticles ; Microfluidic System ; Dielectrophoresis Technique

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