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Designing Polymeric Biomaterials based on Polysaccharides with Tissue Adhesion and Hemostasis Performance

Shokrani, Hanieh | 2023

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 56539 (03)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Kordzadeh, Azadeh
  7. Abstract:
  8. Death can be the result of severe bleeding which is associated with the loss of a high amount of blood like what happens in accidents, wars, or operations. Classic methods to stop bleeding are mostly ineffective to decrease the hemostasis time and the volume of the lost blood. A wide variety of products like powders, foams, and hydrogels have been designed and synthesized either from natural or synthetic polymers. However, limitations like weak tissue adhesion, creating a moist environment in the wound site, infection, weak biodegradation, and hemolysis still have remained. Among the existing options to be used as hemostasis agents, polysaccharides can be appropriate considering their great biocompatibility, their ability to concentrate platelets due to high blood absorption as well as the possibility to be used as hydrogels adjacent to other materials. In this study, we used carboxymethyl cellulose as a polymer, and montmorillonite as a nano additive both of which have hemostasis performance. Plus, we utilized iron ions as electrostatic crosslinkers as well as dopamine to induce antibacterial and tissue adhesion properties. Our optimized sample included 5% carboxymethyl cellulose, 1% montmorillonite, 3% iron ions, and 0.6% dopamine. Blood clotting index and clotting time were respectively 0.37 and 21 seconds for the optimized sample. Plus, biodegradation was 68% within 14 days which is appropriate. The swelling ratio was also 2.5 which indicates the high capability of hydrogel to absorb blood and concentrate platelets. Antibacterial efficacy was also evaluated for both gram-positive (S. aureus) and gram-negative (E. coli) bacteria and results indicated that the hydrogel had a better antibacterial performance on gram-positive bacteria with the inhibition zone of 42 mm. A hemolysis test was also performed to evaluate the hemocompatibility of the hydrogel and a hemolysis ratio of 2% was reported which is promising
  9. Keywords:
  10. Biomaterials ; Cellulose ; Nanomaterials ; Hydrogel ; Carboxymethyl Cellulose ; Montmorillonite ; Antibacterial Properties ; Hemostasis

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