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Application of Advanced Oxidation Processes and Biological Methods to Degrade Levofloxacin

Solouki, Melika | 2024

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 56917 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Yaghmaei, Soheila; Ghasemian, Saloumeh; Ghobadinejad, Zahra
  7. Abstract:
  8. Levofloxacin has been widely used in recent years as an antibiotic in the treatment of various bacterial infections. This drug compound is known as a newly emerging contaminant. In this study, the degradation of this pollutant has been investigated using advanced oxidation processes, biological process, and a combination of advanced oxidation-biological processes. In order to evaluate the degradation of levofloxacin with advanced oxidation processes, an electrochemical process was used. Advanced oxidation processes were carried out to optimize initial concentration parameters of the contaminant (5-15 milligrams per liter), electrolyte concentration (2.5-10 grams per liter), and current density (20 -1 milliampere per square centimeter) over a period of 90 minutes, and based on the results obtained, optimal parameters for initial concentration of levofloxacin, electrolyte concentration, and current density were determined to be 5 milligrams per liter, 7.5 grams per liter, and 16 milliamperes per square centimeter respectively. The results showed that 69% of levofloxacin was degraded under optimal conditions within 90 minutes. Using liquid chromatography-mass spectrometry analysis, the presence of some compounds such as toluene, xylene, and benzaldehyde resulting from the electrochemical method was reported. For biological treatment investigation, the extent of biological degradation of levofloxacin using a strain belonging to Enterobacter cloacae was studied. During biological treatment, levofloxacin with an initial concentration of 5 milligrams per liter was removed by 21% after 5 days and by 36% after 10 days. Since each of the biological and advanced oxidation processes has its own specific drawbacks, in this study a combination of both methods was also investigated. For this purpose, biological degradation of the effluent flow from the electrochemical cell in the first stage using a strain belonging to Enterobacter cloacae was studied. The results showed that by employing both methods, the degradation rate of levofloxacin with an initial concentration of 5 milligrams per liter will be 90% after 5 days. It was also observed that naphthalene dioxygenase and manganese peroxidase enzymes are active in the biological degradation of levofloxacin. Analysis of results using liquid chromatography-mass spectrometry led to identification of some intermediate products in water and introduction of a possible pathway for biological degradation of levofloxacin by selected strain
  9. Keywords:
  10. Levofloxacin ; Electrochemical Treatment ; Advanced Oxidation Process ; Laccas Enzyme ; Naphthalene Dioxygenase Enzyme ; Manganese Peroxidase

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