Loading...

Design, Fabrication and Optimization of a Biodegradable Scaffold Based on Smart Polymer for Application in Tissue Engineering

Hosseinian, Samira | 2024

0 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 57125 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir; Aryanpour, Masoud
  7. Abstract:
  8. As a connective tissue devoid of blood vessels, lymphatics, and nerves, articular cartilage has limited self-repair capabilities. This is because essential nutrients and signals necessary for cell proliferation are restricted. Despite advances in therapeutic approaches, complete repair of damaged cartilage remains a major challenge in medicine. Tissue engineering has attracted considerable attention as a novel approach for the repair of damaged tissues, including cartilage. In this study, a temperature-sensitive and injectable hydrogel based on chitosan-bacterial nanocellulose and silk fibroin was designed and optimized for controlled release of quercetin. After selecting a constant ratio of silk fibroin, different ratios of chitosan and bacterial nanocellulose were selected as a substrate for transporting curcumin-containing microspheres. Various properties of the hydrogel, such as gelation percentage and time, water uptake capacity, porosity percentage, biodegradability, and mechanical strength were evaluated. Finally, the drug release characteristics, antibacterial properties, and biocompatibility of the optimized hydrogel with quercetin microspheres were assessed. The results showed that the hydrogel with 3 and 4 volume ratios of chitosan to bacterial nanocellulose had better performance in the experiments and was selected as the optimized sample. This hydrogel had a suitable pore size (about 122 μm) and water absorption capacity up to 16 times its dry weight. Although the hydrogels with 5 and 2 ratios of chitosan to bacterial nanocellulose showed better biocompatibility, the optimized hydrogel also had no toxicity for cells and its controlled degradation rate (about 50% after 30 days) provided the necessary conditions for controlled release of quercetin and tissue repair. The chitosan-bacterial nanocellulose temperature-sensitive hydrogel along with silk fibroin-dodecyl sulfate shows considerable potential as a scaffold for controlled release of quercetin in cartilage repair. This system can be investigated as a novel approach for the treatment of cartilage injuries in future studies
  9. Keywords:
  10. Cartilage Tissue Repair ; Chitosan ; Quercetin Drug ; Silk Fibroin ; Controlled Release ; Thermosensitive Hydrogels ; Bacterial Nanocellulose ; Biodegradable Scaffold

 Digital Object List

 Bookmark

No TOC