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Design and Fabrication of Peptide-Based Hybrid Hydrogel for Spinal Cord Injury Repair

Raei, Faezeh | 2024

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 57213 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Ramezanpour, Soruor
  7. Abstract:
  8. Spinal cord injury is a systemic disease related to the central nervous system and one of the most severe traumatic diseases that affects a large number of people each year. Inadequate control of the inflammation at the site of injury and the lack of a suitable biomimetic environment are two major and significant factors in the further expansion of the damage. In this research, a polymer/peptide hybrid hydrogel was prepared using a combination of high molecular weight hyaluronic acid and a self-assembling peptide with the IKVAV biological epitope derived from nerve tissue laminin. To create reversible interactions between the polymer and peptide, hyaluronic acid chains were modified with fluoronylmethoxycarbonyl (Fmoc), and a Fmoc based peptide was utilized. The presence of the peptide in the hybrid hydrogel, not only led to a change in the secondary structure of the peptide from a random coil to a structure similar to alpha helices; but also resulted in a 73% improvement in the rheological properties of the hydrogel. The formation of dynamic and reversible π-π bonds between the polymer and peptide led to the development of an injectable hydrogel with suitable self-healing properties. Additionally, cell cytotoxicity and attachment was evaluated using the PC12 cell line. The results showed that the presence of the peptide increased cell viability by 15% and caused elongation of cells compared to the sample without the peptide respectively. Finally, the drug release from the hydrogel was investigated for 24 hours using the anti-inflammatory drug prednisolone to control the initial neuroinflammation at the injury site. In general, it can be concluded that the presence of the peptide leads to the improvement of the physical and biological properties of the hydrogel, making it a suitable choice for use in spinal cord injuries
  9. Keywords:
  10. Hyaluronic Acid Gel ; Self Assembled Peptide Nanoparticle (SAPN) ; Injectable Hydrogel ; Nerve Tissue Engineering ; Hybrid Self-Healing Hydrogel ; Prednisolone ; Spinal Cord Injury

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