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Fabrication and Characterization of Chitosan/Gelatin Based Scaffolds Using Adipose Derived Stem Cells Applicable in Skin Tissue Engineering

Dorri Nokoorani, Yeganeh | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 58114 (08)
  4. University: Sharif University of Technology
  5. Department: Civil Engineering
  6. Advisor(s): Shamloo, Amir; Firouzbakhsh, Keikhosrow
  7. Abstract:
  8. Skin tissue engineering, is considered as a method to heal full-thickness wounds. The basis of this method is fabrication a substrate named scaffold in order to replace the lost skin tissue temporarily. The aim of the present study is the fabrication and characterization of skin scaffolds using two natural polymers, which are Chitosan and Gelatin. This study is divided into three phases: first is the investigation of the influence of two cross-linkers named Glutaraldehyde and a kind of carbodiimide on the properties of scaffolds, second is the characterization of scaffolds fabricated by three different ratios of chitosan/gelatin and third is the evaluation of the effect of Allantoin on chitosan/gelatin scaffolds. In the present study, several characterization methods are utilized in order to investigate the properties of scaffolds, such as scanning electron microscopy, swelling ratio, degree of cross-linking, uni-axial tensile test, rheological properties, anti-bacterial experiment, cell viability, cell adhesion, degradation rate, FTIR analysis and drug release rate. Besides, a study on adipose derived stem cells cultured on the scaffolds was performed in collaboration with Dermatology Research Center of Shohadaye Tajrish Hospital. The results of the first phase of this study reveal that cross-linking the scaffolds using 0.3% w/v of carbodiimide type cross-linker, leads to 88 percent cross-linking and is the best way to construct scaffolds in the current study. The results of the second phase, show that the constructed Chitosan/Gelatin scaffold with the ratio of 1:1, with pores around 275 micro meters, degradation rate about 75 percent in 21 days, young modulus equal to 0.0289 MPa, swelling ratio around 5 times greater than the initial weight of the scaffold in 150 minutes and cell viability about 50 percent is the most suitable drug free scaffold investigated in the present study. Furthermore, the investigations show that addition of Allantoin into scaffolds, results in changing the properties of the substrates such as decreasing degree of cross-linking, increasing water absorption, reducing ultimate tensile strength and increasing the antibacterial properties. The overall conclusion of this section, is selecting the scaffold containing 0.5% w/v Allantoin
  9. Keywords:
  10. Skin Tissue Engineering ; Gelatin ; Chitosan ; Allantoin ; Freeze Drying Method ; Stem Cell ; Adipose Derived Stem Cells ; 1-Ethyl-3-(3-dimethylaminopropyl)Carbodiimide ; EDC-NHS Coupling Reagents

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