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    Co-Microencapsulation of Folic Acid and Iron

    , M.Sc. Thesis Sharif University of Technology Aali, Fatemeh (Author) ; Alemzadeh, Iran (Supervisor) ; Vossough, Manouchehr (Supervisor)
    This study aimed to prepare a type of microcapsule that microencapsulates folic acid and iron at the same time and protects these two substances against environmental and gastrointestinal conditions. Simultaneous release of these two nutrients has dual health properties and improves the efficiency of folic acid microencapsulation. In this study, biocomposites were formed containing alginate, pectin, and carboxymethylcellulose, and the microcapsules were synthesized by the ionization of calcium-alginate ions. Biocomposite optimization mixture with 13 experiments using experimental design software, optimal combination design method based on repopulation separator, restoring folic acid... 

    Co-microencapsulation of Probiotic Bacteria and DHA Fatty Acid; Optimization and Evaluation of the Viability of Probiotic and Oxidative Stability of DHA during Gastrointestinal Tract

    , M.Sc. Thesis Sharif University of Technology Vaziri, Asma Sadat (Author) ; Alemzadeh, Iran (Supervisor) ; Vossoughi, Manouchehr (Supervisor)
    Due to the increased public awareness about the role of food on health, an increased interest of consuming fortified food has grown rapidly. Probiotic bacteria and DHA poly-unsaturated fatty acid are two major bioactive ingredients becoming increasingly popular in food fortification industry due to their beneficial effects. However, high susceptibility of probiotic bacteria during production, maintenance and digestion in acidic and enzymatic conditions of gastrointestinal tract results in decreasing the survivability and therefore reducing health effects in body. Besides, having multiple double bonds in the structure of DHA fatty acid leads to oxidative degradation that produces volatile... 

    Stem Cell Proliferation and Differentiation in Bioreactors

    , M.Sc. Thesis Sharif University of Technology Rezaei, Maryam (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    In this study, differentiation of rabbit embryonic-derived mesenchymal stem cells to osteogenic cells has been characterized. Bone tissue engineering is based upon the understanding of bone tissue construct and it’s formation in-vivo, and the Preparation of tissue engineered bone constructs to repair large size defects is it’s major goal. We sought to investigate the combined effect of three elemnts of tissue engineering: cells, scaffolds and growth factors. Mesenchymal stem cells are unspecialized cells which due to their unlimited self-renewal capacity and the remarkable ability to differentiate along multiple linage pathways are natural choice for application in tissue repair and... 

    Comparing Effects of Natural Antibiotics and Anti Bacterial Materials in Burn Wound Infections with Nanoparticles and Skin Scaffold

    , M.Sc. Thesis Sharif University of Technology Ramezani, Bita (Author) ; Alemzadeh, Iran (Supervisor) ; Vosoughi, Manouchehr (Supervisor)
    Burns are one of the most important accidents related to human health. Due to the intense physical and mental complications and high fatality rate associated with them, receiving proper treatment is of paramount importance. The control of infection in wounds would cure and eliminates the effect of wounds and treatment of skin lesions with engineered scaffolds can be an effective method. The purpose of this project is proposing a hydrogel scaffold based on natural polymers of oxidized alginate and gelatin loaded with an herbal drug to control infection and treat burn wounds. For this purpose, the Iranian Oak extract that it's main content is Tanin and PolyPhenolinc materials, was prepared and... 

    Study, Optimization and Construction of a Microfluidic Gene Amplification Device by Using Thin Film Layer Method

    , M.Sc. Thesis Sharif University of Technology Eslami, Sara Sadat (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Co-Supervisor) ; Shamloo, Amir (Co-Supervisor)
    Polymerase Chain Reaction (PCR) is a process in which a special piece of a gene is amplified millions of times over a short period. This method has been of paramount importance in different fields of research and has been applied for different applications. PCR requires thermal cycling, or repeated temperature changes between two or three discrete temperatures to amplify specific nucleic acid target sequences. To achieve such thermal cycling, conventional bench-top thermal cyclers generally use a metal heating block powered by Peltier elements or benefit from forced convection heat transfer. Due to the fact that these methods are time consuming, it seems that design and fabrication of a fast... 

    Study & Synthesis of Biocompatible Scaffolds for Animal Cell Cultures

    , M.Sc. Thesis Sharif University of Technology Riazi Esfahani, Bahar (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Co-Advisor) ; Pajoum Shariati, Ramin (Co-Advisor)
    Natural tissues of body consist of Extra Cellular Matrix (ECM) and cells. Scaffold produces a temporary matrix for cells and facilitates oxygen and nutrients transport which enhances tissue regeneration. Therefore, attempts in regeneration of tissues and organs by scaffolds attracted great interest in today’s researchs. Choosing scaffold fabrication method and its polymer type, play a key role in scaffold specifications as they determine scaffold properties. In this project electrospinning was chosen as the scaffold fabrication method which is because of the unique properties of scaffolds fabricated by this method and also its ability in producing micro and nano fibers. At first set of... 

    Application of Ultrasonic Technique to Improve Food Processing and Yield Enhancement in Food Industry

    , M.Sc. Thesis Sharif University of Technology Halalipour, Ali (Author) ; Roosta Azad, Reza (Supervisor) ; Aalemzadeh, Iran (Supervisor) ; Ghotbi, Siroos (Supervisor)
    In this project we focused on Ultrasonic waves as novel, effective and well-controllable means for cell wall disruption and disintegration to provide faster and more complete penetration of solvent into cellular materials and improve sugar mass transfer and extraction from sugarcane and sugar beet. Impact of frequency, temperature, power density and reactor shape on Ultrasonic wave efficiency in extraction process has been investigated and the result was utilized to design a semi-pilot reactor. In the optimum situation, sucrose extraction from sugarcane improved to 4.51 gr/100 ml H2O that had a significant increasing from 2.1 gr/100 ml H2O extraction in thermal only process at 70 °C. Optimum... 

    Study and Synthesis of Biocompatible Polymer and Loading of Peptide Drug for using in Drug Delivery

    , M.Sc. Thesis Sharif University of Technology Nikravesh, Niusha (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Co-Advisor)
    With the rapid development of biotechnology, peptide and protein drugs are now playing an increasingly important role in therapeutics. Compared with chemical drugs, peptide and protein drugs have some limitations such as low stability and rapid deactivation. Biodegradable and biocompatible polymeric micro carriers have been shown to have a high potential for the delivery of peptides and proteins. Among these polymers, alginate has been widely investigated as a biomaterial. Alginates are natural polysaccharide polymers isolated from brown seaweed. Bovine serum albumin (BSA) loaded calcium alginate microspheres produced in this study by a modified w/o emulsification method. The influence of... 

    Study and Fabrication of a Multilayer Scaffold Containing Biological Agents for Skin Wounds Regeneration

    , Ph.D. Dissertation Sharif University of Technology Hajiabbas, Maryam (Author) ; Alemzadeh, Iran (Supervisor) ; Vossoughi, Manouchehr (Supervisor) ; Shamloo, Amir (Co-Supervisor)
    In recent years, it is expected that the fabrication of multilayer scaffolds and the use of different methodologies in one product can be a new progressing method in skin substitute production. Accordingly, this project aims to fabricate a bilayered composite scaffold with a combination of hydrogel and electrospinning method. We have tried to prepare a scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) without using corrosive solvents and toxic crosslinking agents as a scaffold and drug delivery system. As different biological, chemical, physical, and mechanical factors play a vital role in the healing process, we have characterized the proposed scaffold via DSC,... 

    Fabricate of Novel Drug Vector Based on Metallic Nanoparticles and Study of its Effects on Cell Line

    , Ph.D. Dissertation Sharif University of Technology Asadishad, Bahareh (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    Application of nanoparticles in targeted delivery and controlled drug release is recently highly attracted especially in cancer therapy. In this study with the aim of developing a novel drug vector based on metallic nanoparticles, gold nanoparticles with their unique characteristics were used to design a vector for targeted anti-cancer drug delivery to tumor. First, gold nanoparticles were chemically synthesized therough sodium citrate and sodium brohydrate reductions with average diameters of 22 and 11 nm, respectively. Then, the activated poly ethylene glycol (PEG) and activated folic acid were conjugated to the lysine-capped gold nanoparticles. Subsequently, the widely used anticancer... 

    Study and Fabrication of a Microfluidic System to Investigate Stem Cell Differentiation

    , M.Sc. Thesis Sharif University of Technology Motmaen Esfahani, Shayan (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    In recent years, many studies have conducted research about stem cells due to their prominent features such as proliferation and differentiation. By changing the specific parameters of the cellular micro-environment, it is possible to determine the stem cells fate and thereby use them in medical affairs. For this purpose, various devices and methods have been designeds such as microfluidic devices and scaffolds. The microfluidic devices has been especially focuced in the researchs due to their unique features, like the possibility of testing with small amounts of samples, the creation and control of low cost cellular micro-environment and low practical time. Two-dimensional scaffolds made up... 

    Study of a Biosensor Based on Nanofibers

    , M.Sc. Thesis Sharif University of Technology Shamloo, Arezoo (Author) ; Vossoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    In this project an enzymatic peroxidase biosensor based on two naono structured material 1-Polyaniline nananofibers 2-Polyethylene glycol-Poly citric acid copolymer was studied for phenol detection.Polyaniline nanofibers and poly ethylene glycol-poly citric acid copolymer were synthetized by chemical polymerization.Electrochemical behaviors of these biosensors were studied using cyclic voltammetry (CV) and amperometric techniques. The amperometric response of the coated electrodes is highly stable with 92% of the initial activity remaining after 5 determinations. These phenol biosensors show an oxidation peak at 0.55V. The amperometric response for phenol biosensor based on Polyaniline... 

    Studies on DBT Removal from Compositional Oil Model Using CNT

    , M.Sc. Thesis Sharif University of Technology Imani, Masoumeh (Author) ; Vossoughi, Manoochehr (Supervisor) ; Aalemzadeh, Iran (Supervisor)
    In this research, carbon nanotubes (CNTs) and CNT coated by titanium dioxide (TiO2) were used as adsorbents for desulfurization. Photocatalytic oxidation by titanium dioxide Degussa P-25 nanoparticles (as photocatalyst) immobilized on a porous and low-density support called “perlite” and CNT/TiO2 was perused. TiO2-coatings were prepared by liquid phase deposition method.This is a wet process for the formation of metal oxide thin films on substrates. The coating of nanotubes with TiO2 was confirmed by IR and EDAX and morphological properties were observed by SEM analysis.The physical properties of adsorbents were determine using BET.Dibenzothiophene (DBT)dissolved in acetonitrile was used as... 

    Experimental Study of Biological Synthesis, Stabilization and Delivery of the Recombinant Protein of Chondroitinase

    , Ph.D. Dissertation Sharif University of Technology Askaripour, Hossein (Author) ; Vossoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor) ; Khajeh, Khosro (Supervisor)
    In this research, immobilization method was employed to increase the thermal stability of Chondroitinase ABC I (cABC I) enzyme. In first step, magnetite nanoparticle (Fe3O4) was selected as a support and cABC I enzyme was attached via the adsorption method. The results showed that pH=6.5, temperature 15 ˚C, enzyme-to-support mass ratio 0.75, and incubation time 4.5 hr were the appropriate conditions for immobilizing cABC I enzyme on Fe3O4 nanoparticle. It was also found that the optimum pH for free and immobilized enzymes was 7.6 and 8.0, respectively. The maximum activity of free enzyme was obtained at 25 ˚C, whereas the activity of immobilized enzyme was almost constant in the temperature... 

    Empirical Study for Removal of Oil Contamination by Thermophilic Bacteria

    , M.Sc. Thesis Sharif University of Technology Babaeivelni, Kamel (Author) ; Vossoughi, Manuchehr (Supervisor) ; Alemzade, Iran (Supervisor)
    Biodegradation has always been as a proper method for treatment of oil contamination. Accordingly, several investigations have been done by mesophilic bacteria for biodegradation of hydrocarbons while few studies have been conducted about thermophilic bacteria.
    There are many factors like high growth rate of thermophilic bacteria and good resistance of enzymes secreted from thermophiles against physical and chemical variations lead to the extensive use of thermophilic bacteria for removal of oil contamination. The important factors should be optimized in order to choose a suitable method for the removal of oil contaminations. Optimal conditions for oil contamination removal by... 

    Experimental Investigation and Modeling of Ferrous Biooxidation in a Monolithic Bioreactor

    , Ph.D. Dissertation Sharif University of Technology Kahrizi, Ehsan (Author) ; Vossoughi, Manoochehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    The bio-oxidation of ferrous iron is a potential industrial process in the regeneration of ferric iron and the removal of H2S in combustible gases. Bio-oxidation of ferrous iron may be an alternative method of producing ferric sulfate, which is a reagent used for removal of H2S from biogas, tail gas and pulp and paper industry. For practical use of this process, this study evaluated the optimal pH and initial ferric concentration. pH control looks like a key factor as it acts both on growth rate and on solubility of materials in the system. Some process variables such as pH and amount of initial ferrous ions on oxidation by A. ferrooxidans and the effects of such process variables dilution... 

    Experimental Studies and Optimization of Microalgae Cultivation in a Photo-Bioreactor for Bio-Diesel Production

    , M.Sc. Thesis Sharif University of Technology Abdollazadeh, Himan (Author) ; Vossoghi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)

    Experimental Study and Optimization of Bioremediation of PAHs by Newly Isolated Thermophilic Filamentous Fungi in Slurry Bioreactor

    , M.Sc. Thesis Sharif University of Technology Sarayloo, Ehsan (Author) ; Vossoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    Sustainable development requires the development and promotion of environmental management and a constant search for green technologies to treat a wide range of aquatic and terrestrial habitats contaminated by increasing anthropogenic activities. In this work we investigated the ability of four previously isolated fungi, BBRC 9057, BBRC 20020, BBRC 9058 and BBRC 9002, in degradation of polycyclic (PAHs) and the effect of UV irritation on fungi cell growth and degradation of PAHs. Also we studied the degradation of anthracene by thermophilic filamentous fungi in liquid and soil slurry bioreactor. Cell growth and anthracene degradation ability of BBRC 9057 increased 6.51 and 1.82 times rather... 

    Biofuel Alcohol Production from Agricultural Residues in a Slurry Bioreactor

    , M.Sc. Thesis Sharif University of Technology Sattari, Behzad (Author) ; Vossoughi, Manoochehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    Nowadays bioethanol is one of the main factors in the fuel market. It is currently produced from sugars and starchy materials, but lignocelluloses can be expected to be major feedstocks for ethanol production in the future. Agricultural residues such as wheat straw are being considered a potential lignocellulose raw material for fuel-ethanol production as an alternative to starch or sugar-containing feedstock. In order to produce bioethanol, it is necessary to decompose the wheat straw into soluble sugars. But lignocellulosic materias needs pretreatment before hydrolysis and fermentation. The main goal of pretreatment is to increase the enzyme accessibility improving digestibility of... 

    Experimental Studies for Construction of a Microbial Fuel Cell (MFC) in Continuous Flow Mode

    , M.Sc. Thesis Sharif University of Technology Sadeghi Haskoo, Mohammad Amin (Author) ; Vossoughi, Manoochehr (Supervisor) ; Aalemzadeh, Iran (Supervisor)
    In this research performance of microbial fuel cells (MFCs) in continuous flow mode was studied. Different anodic chambers were tested and it was found that granular activated carbons (GACs) produced the highest power density (1228 mW/m3) in comparison with multiple anodes (731 mW/m3), single anode (294 mW/m3) and polymeric packings (40 mW/m3). It was also shown that in a plug-anodic chamber (PAC) the power output is reduced by reducing agitation of anodic volume. Adding more GACs to anodic chamber results in power increase, however by increasing occupied volume from 80% to 100% the power increase was negligible in result of cathodic reactions limitations. Feed flowrate was increased from...