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dextrances
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Synthesis of Dextran-MA-Gly-Gly-NHNH2 Copolymer as a Possible Biodegradable and pH-responsive Carrier for Doxorubicin
, M.Sc. Thesis Sharif University of Technology ; Pourjavadi, Ali (Supervisor)
Abstract
Some chemotherapy drugs have unpleasant side effects and use them due to the instability and low solubility in blood flow is associated with problems and limitations.To solve these problems, controlled drug delivery systems have been proposed. In this process, the polymer increased solubility, stability and low toxicity and the active drug realease in a particular tissue or controlled speed. Therefore be less adverse side effects. Dextran is the one of biocompatible and water-soluble polysaccharides that due to its unique characteristics, widely used as carriers in drug delivery. Dextran molecules can bind to drugs and increased solubility, stability, and reduce their side effects....
Experimental Study of Biological Synthesis, Stabilization and Delivery of the Recombinant Protein of Chondroitinase
, Ph.D. Dissertation Sharif University of Technology ; Vossoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor) ; Khajeh, Khosro (Supervisor)
Abstract
In this research, immobilization method was employed to increase the thermal stability of Chondroitinase ABC I (cABC I) enzyme. In first step, magnetite nanoparticle (Fe3O4) was selected as a support and cABC I enzyme was attached via the adsorption method. The results showed that pH=6.5, temperature 15 ˚C, enzyme-to-support mass ratio 0.75, and incubation time 4.5 hr were the appropriate conditions for immobilizing cABC I enzyme on Fe3O4 nanoparticle. It was also found that the optimum pH for free and immobilized enzymes was 7.6 and 8.0, respectively. The maximum activity of free enzyme was obtained at 25 ˚C, whereas the activity of immobilized enzyme was almost constant in the temperature...
Synthesis and Characterization of Dextran Based Magnetic Nanogels as a Gene Delivery Vector and Investigating its Gene Therapy Efficiency
, M.Sc. Thesis Sharif University of Technology ; Madaah Hosseini, Hamid Reza (Supervisor) ; Arefian, Ehsan (Supervisor)
Abstract
The use of magnetic nanoparticles modified with proper surface agent and the ability of controlling by magnetic field, that can induce colloidal stability, is considered as a vector to transfer DNA plasmid, or pDNA in short, in the field of gene therapy. In thiss research, pH-responsive dextran-based magnetic nanogels (dextMNGs) were synthesized via inverse mini-emulsion method. Fourier transformation infrared spectroscopy (FTIR) showed that magnetite nanoparticles (MNPs) were successfully modified with arginine and had amine terminals. FTIR, also, proved that aldehyded dextran was crosslinked by arginine modified magnetite nanoparticles (RMNPs) via pH sensitive imine bonds. X-ray...