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Production Study of Protease Enzyme by Immobilized Cells

Sabzevari, Mohammad Amin | 2010

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 40859 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Yaghmaei, Soheila; Kazemi, Akhtarelmolook
  7. Abstract:
  8. Microbial proteases are among the most important hydrolytic enzymes and havebeen studied extensively since the advent of enzymology. They are essentialconstituents of all forms of life on earth, including prokaryotes, fungi, plants andanimals.In recent years there has been a phenomenal increase in the use of alkaline protease asindustrial catalysts. Alkaline proteases (EC.3.4.21–24, 99) are defined as thoseproteases which are active in a neutral to alkaline pH range. Cell immobilization is a technique that restricts the free movements of microorganisms in the process and it has a lot of advantages including this fact that biotechnologists’ dream for using continuous system in fermentation processes, comes true. In the present work this technique has been used for alkaline protease production. Different immobilization methods exploited including Sodium alginate micro-encapsulation, Chitosan, Corn Cob and Corn Tassel adsorption.
    Considering the reaction condition, Corn Cob with 60% immobilization capacity and the highest enzyme activity, chosen as the best carrier among all the others,Then production condition was modified using Corn Cob as carrier. The modified time that cell suspension and Corn Cob mixed under modified condition, was 5 hr and the modified speed for shaker incubator was 80 rpm. We could not use the micro-encapsulation technique because of some limitations in bioreaction conditions. Alginates, the natural polymer that widely used for cell entrapment, is soluble in Phosphate buffer and pH = 7-8. Unfortunately hardening techniques did not work.
  9. Keywords:
  10. Alkaline Protease ; Cell Immobilization ; Chitosan ; Sodium Alginate ; Corn Cob ; Corn Tassel

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