Human Cells Separation Via Imprinted Polymer

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Sabaghi, Davood | 2013

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 44866 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Abdekhodaie, Mohammad Jafar
Abstract:
The goal of this research is to elucidate the mechanism of Cell recognition in molecularly imprinted polymers (MIPs) using already utilized techniques. Our approach employs a more flexible non-covalent imprinting method which starts from a readily available polymer and utilizes an aqueous environment for both MIP synthesis and testing. Cell MIPs against MG-63 cell were synthesized. The synthesis procedure was optimized to obtain better binding characteristics to the targeted cell. Adsorption of target cell onto imprinted Alginate Spheres was facilitated by these macromolecular fingerprints as revealed by various microscopical examinations The imprinted Spheres showed high selectivity toward each of test cell with high adsorption affinity making them excellent candidates for rapid detection of cell from liquids
Keywords:
Cell Imprinting ; Alginate ; Cell Separation ; Human Cells ; Cancer Cells

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