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Removal of chlorophenolic derivatives by soil isolated ascomycete of Paraconiothyrium variabile and studying the role of its extracellular laccase

Forootanfar, H ; Sharif University of Technology | 2012

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  1. Type of Document: Article
  2. DOI: 10.1016/j.jhazmat.2012.01.012
  3. Publisher: 2012
  4. Abstract:
  5. The ability of Paraconiothyrium variabile, a laccase producing ascomycete recently isolated from soil, was studied to eliminate chlorophenol derivatives in submerged culture medium. Among the tested compounds, ρ-chlorophenol (ρ-CP) and pentachlorophenol (PCP) were found to have minimum and maximum toxic effects, respectively, on the growth of the microorganism and at the same time high and low bioelimination percentages. The fungal strain was able to remove 86% of ρ-CP (with initial concentration of 40mgl -1) and 56% of 2,4-dichlorophenol (2,4-DCP; with same concentration as ρ-CP) after 9 days of incubation while no elimination was observed in the presence of 2,4,6-trichlorophenol (2,4,6-TCP) and PCP. Monitoring of laccase production level in the fermentation broth together with pollutant removal confirmed the key role of this copper-containing oxidase in chlorophenol derivatives elimination. The type of laccase inducer (guaiacol) and its final concentration (250μM) and also initial pH of the fermentation broth (pH=5.5) in the elimination of ρ-CP increased the final removal yield from 86% to 94.3%
  6. Keywords:
  7. Pollutant ; 2 ,4 ,6-Trichlorophenol (2 ,4 ,6-TCP) ; 2 ,4-DCP ; 2 ,4-Dichlorophenol ; Chlorophenol derivatives ; Extracellular ; Fermentation broths ; Fungal strains ; Initial concentration ; Initial pH ; Laccase production ; Laccases ; Pollutant removal ; Submerged cultures ; Toxic effect ; Phenols ; Removal ; Fungi ; 2,4,6 trichlorophenol ; Chlorophenol ; Guaiacol ; Oxidoreductase ; Pentachlorophenol ; Chemical pollutant ; Chlorophenol ; Copper ; Enzyme activity ; Fungus ; Growth rate ; Mycoparasite ; Toxicity test ; Ascomycetes ; Controlled study ; Culture medium ; Fungal strain ; Nonhuman ; Paraconiothyrium variabile ; pH ; Soil ; Ascomycota ; Biodegradation, Environmental ; Culture Media ; Fermentation ; Hydrogen-Ion Concentration ; Laccase ; Soil Microbiology ; Paraconiothyrium
  8. Source: Journal of Hazardous Materials ; Volume 209-210 , 2012 , Pages 199-203 ; 03043894 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0304389412000283