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Kinetics, experimental and simulation studies of Chinese Hamster Ovary cell growth in a packed-bed bioreactor

Shakibaie, M ; Sharif University of Technology | 2011

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  1. Type of Document: Article
  2. Publisher: 2011
  3. Abstract:
  4. To figure out the relationship between nutrient deprivation and cell growth, simulation can be effective. In the present work, the growth of Chinese Hamster Ovary (CHO) cells was simulated based on stoichiometric and kinetics calculations. The simulation results were compared to the experimental data from a packed-bed bioreactor and a good agreement was observed. The kinetic parameters (K Glc, K Amn, K Lact and μ Max) were optimized by a genetic algorithm method using stoichiometric parameters (Y X/Glc, Y X/Amm, and Y Lac/Glc). The stoichiometric and kinetic parameters were used in the simulation to study the growth of CHO cells. The concentrations of the toxic by-products, ammonium and lactate, were measured at different values of dissolved oxygen, 30% and 50% of saturated air. While dissolved oxygen was maintained at 30% of saturation, the maximum that ammonium and lactate concentrations reached were 0.099 and 2.1 g/l, respectively. At dissolved oxygen level of 50% air saturation, the maximum ammonium and lactate concentrations decreased to the values of 0.082 and 1.8 g/l, respectively. The cell density of 22×10 8 cells/l was achieved after 120 h of cultivation at dissolved oxygen of 50% in a packed-bed bioreactor
  5. Keywords:
  6. CHO cells ; Kinetics ; Packed-bed bioreactor ; Simulation ; Stoichiometry
  7. Source: World Applied Sciences Journal ; Volume 15, Issue 11 , 2011 , Pages 1568-1575 ; 18184952 (ISSN)
  8. URL: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.389.8101