Experimental Study on Biosensors for Detection of Biohazards with Nano-structured Metal Oxides Basis

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Dadfarmay, Sajad | 2016

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 50024 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Mashayekhan, Shohreh; Vosoughi, Manouchehr
Abstract:
In this research, fabrication of a mediator free nano-biosensor for the detection of cyanide has been studied. This biosensor utilizes 75 nm diameter (nano) ZnO particles synthesized with sol- gel method for modification and immobilization of Horseradish peroxidase (HRP). Cyanide plays inhibitory role in decomposition of H2O2 while HRP plays catalyst role in this reaction. Cyanide inhibits enzyme activity thorough binding with enzyme active sites and result in decreasing electrical current in electrochemical decomposition of H2O2. Amperometery response of biosensor shows linear relation between inhibition percent and cyanide concentration in range of 3µM - 24µM with detection limit of 0.62µM and R2=0.98 (n=8). pH, H2O2 concentration and applied potential have great effect on inhibition percent, thus optimized values for these parameters were obtained
Keywords:
Hydrogen Peroxide ; Cyanide ; Peroxidase Enzyme ; Biosensor ; Amperometric Method ; Nanozinc Oxide

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