Loading...

Effect of cell concentration on the acylation of penicillin G enzymatic reaction in immobilized cells

Barin, M ; Sharif University of Technology | 2009

1217 Viewed
  1. Type of Document: Article
  2. Publisher: 2009
  3. Abstract:
  4. E.coli was immobilized by entrapment within calcium alginate beads using cell suspensions of different concentrations. The immobilization procedure resulted in beads with a homogeneous cell distribution, referred to as a Non-overlapping Cell (NC) configuration. If cells within the beads were allowed to grow, the NC configuration would transform into a Clustered Cell (CC) configuration as a, result of cell growth. Enzyme activity and substrate conversion were obtained for NC and CC configurations of different cell density, using penicillin G acylation by penicillin G acylase, to produce. 6-amino penicillanic acid. Enzyme activity and conversion were found to depend on both cell concentration and configuration. Beads with NC configuration resulted in higher enzyme activity and conversion compared with CC configurations of similar cell density. © Sharif University of Technology, June 2009
  5. Keywords:
  6. Cell distribution ; Immobilized cells ; Penicillin G acylation ; Calcium alginate beads ; Cell concentrations ; Cell density ; Cell distribution ; Cell Suspension ; E-coli ; Enzymatic reaction ; Overlapping cells ; Penicillin G ; Penicillin G Acylase ; Substrate conversion ; Acylation ; Carboxylic acids ; Cell culture ; Cells ; Drug products ; Enzyme activity ; Enzymes ; Growth kinetics ; Cell immobilization ; Calcium ; Coliform bacterium ; Immobilization ; Substrate
  7. Source: Scientia Iranica ; Volume 16, Issue 1 , 2009 , Pages 69-73 ; 10263098 (ISSN)
  8. URL: http://scientiairanica.sharif.edu/article_3207.html