Design, Simulation and Fabrication of a Centrifugal Microfluidic Device for Circulating Tumor Cells Separation

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Naghdloo, Amin | 2018

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 50651 (08)
University: Sharif University of Technology
Department: Mechanical Engineering
Advisor(s): Shamloo, Amir; Nouri Borujerdi, Ali
Abstract:
In this project, two centrifugal microfluidic platforms with the goal of separation and genetical study of circulating tumor cells have been designed, simulated and fabricated. Circulating tumor cells are the ones that are detached from the cancer tumors, entered the blood and disseminated the danger of cancer through other parts of the body. Both passive and active methods of separation are investigated in this study. The passive method is based on the inertial effects of the fluid and the active method is based on the magnetophoretic force exerted from an external magnetic field. In order to use the active method, the magnetic nanoparticles are attached to the breast cancer cells by the bonding of cell-specific Ep-CAM antibodies. In addition, the fabricated devices have been tested experimentally in the laboratory in order for their efficiency to be evaluated
Keywords:
Separators ; Circulating Tumor Cells (CTC) ; Nanoparticles ; Centrifugal Microfluidics ; Cancer Diagnosis ; Cancer Cells ; Antibady