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Design and Fabrication of a Microfluidic Chip for Three-dimensional Cancer Cell Culture

Moshksayan, Khashayar | 2018

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 50598 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Saeedi, Mohammad Saeed; Kashaninejad, Navid
  7. Abstract:
  8. Nowadays, we are witnesses of deaths caused by cancer in large statistical amounts. Vast number of investigations are conducted for anticancer drug evaluation on human and animal cells using traditional two-dimensional and three-dimensional culturing platforms. However, most of them are not successful due to the lack of in-vivo relevant microenvironment in these platforms. One of the best cancer in-vitro cultures is the tumor spheroid which is a spherical cellular aggregation. By culturing these tumor spheroids in a microfluidic chip, it is possible to mimic the in-vivo microenvironment elements such as nutrient and metabolite gradients. Here we present a microfluidic device fabricated using photolithography followed by soft lithography of PDMS for formation of tumor spheroids in the microwells embedded in the chip which can be used for high-throughput anticancer drug tests. At first, the device was analyzed numerically to demonstrate its capability of maintaining the suitable oxygen, glucose and shear stress in the culture environment as well as trapping the cells in the microwells. The non-small lung cancer cell line (A549) have been successfully cultured within the chip for five days under culture medium perfusion
  9. Keywords:
  10. Computational Fluid Dynamics (CFD) ; Cancer Cells ; PhotoLithography ; Cell Culture ; Soft Lithographay ; Microfluidic Chip ; Cell Spheroid ; Three Dimentional Cell Culture

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