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A turn-on graphene quantum dot and graphene oxide based fluorometric aptasensor for the determination of telomerase activity

Kazemi, E ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1007/s00604-019-3956-x
  3. Publisher: Springer-Verlag Wien , 2019
  4. Abstract:
  5. A turn-on fluorometric assay is described for determination of the activity of enzyme telomerase. For this purpose, graphene quantum dots (GQDs) were first modified with the telomeric sequence (5′-amino-AATCCGTCGAGCAGAGTT-3′) via a condensation reaction. Injection of graphene oxide causes instant quenching of the blue fluorescence of the GQDs. Addition of cell extract containing telomerase, triggers the extension of telomer via addition of specific sequence (TTAGGG)n to its 3′ end. Fluorescence, best measured at excitation/emission wavelengths of 390/446 nm, is subsequently restored due to folding of the extended telomeric sequence into G-quadruplex structure. The method was applied to the determination of telomerase activity in crude cell extracts of as little as 10 HeLa cells. The linear dynamic range extends from 10 to 6500 cells. [Figure not available: see fulltext.] © 2019, Springer-Verlag GmbH Austria, part of Springer Nature
  6. Keywords:
  7. Biomarker ; Biosensors ; Cancer detection ; Fluorescence ; G-quadruplex ; Nanoprobe ; Optical sensing
  8. Source: Microchimica Acta ; Volume 186, Issue 12 , 2019 ; 00263672 (ISSN)
  9. URL: https://link.springer.com/article/10.1007/s00604-019-3956-x