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GAG positioning on IL-1RI; A mechanism regulated by dual effect of glycosylation

Azimzadeh Irani, M ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1093/glycob/cwz055
  3. Publisher: NLM (Medline) , 2019
  4. Abstract:
  5. IL-1RI is the signaling receptor for the IL-1 family of cytokines that are involved in establishment of the innate and acquired immune systems. Glycosylated extracellular (EC) domain of the IL-1RI binds to agonist such as IL-1β or antagonist ligands and the accessory protein to form the functional signaling complex. Dynamics and ligand binding of the IL-1RI is influenced by presence of the glycosaminoglycans (GAGs) of the EC matrix. Here a combination of molecular dockings and molecular dynamics simulations of the unglycosylated, partially N-glycosylated and fully N-glycosylated IL-1RI EC domain in the apo, GAG-bound and IL-1β-bound states were carried out to explain the co-occurring dynamical effect of receptor's glycosylation and GAGs. It was shown that the IL-1RI adopts two types of "extended" and "locked" conformations in its dynamical pattern, and glycosylation maintains the receptor in the latter form. Maintaining the receptor in the locked conformation disfavors IL-1β binding by burying its two binding site on the IL-1RI EC domain. Glycosylation disfavors GAG binding to the extended IL-1RI EC domain by sterically limiting the GAGs degrees of freedom in targeting its binding site, while it favors GAG binding to the locked IL-1RI by favorable packing interactions. © The Author(s) 2019. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
  6. Keywords:
  7. Glycosaminoglycan ; Glycosylation ; IL-1RI ; Molecular dynamics simulation ; Glycosaminoglycan ; IL1B protein, human ; Interleukin 1 receptor type I ; Interleukin 1beta ; Binding site ; Chemistry ; Glycosylation ; Human ; Metabolism ; Molecular dynamics ; Binding Sites ; Glycosaminoglycans ; Glycosylation ; Humans ; Interleukin-1beta ; Molecular Dynamics Simulation ; Receptors, Interleukin-1 Type I
  8. Source: Glycobiology ; Volume 29, Issue 11 , 2019 , Pages 803-812 ; 14602423 (ISSN)
  9. URL: https://academic.oup.com/glycob/article-abstract/29/11/803/5533449?redirectedFrom=fulltext