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Design and Fabrication of a Microfluidic Device to Study the Growth of Breast Cancer Cell

Mohammad Hashemi, Hanieh | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 53009 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vosughi, Manuchehr; Shamloo, Amir; Naserifar, Naser
  7. Abstract:
  8. for decades Cancer has been one of the major causes of death universally. Though extensive efforts in developing new anticancer therapies, they face failures in clinical trials and curing the sick owing to time consuming and expensive preclinical models with poor predictions of drug responses in human. To address this challenge it is crucial to develop preclinical models mimicking the main aspects of a tumor that can provide effective prediction of therapeutic responses. Tumor on chip technology has appeared as a promising approach for providing effective cancer models and reliable preclinical predictions. In this project, collagen was first extracted as the main constituent of the tumor extracellular matrix. Changes in the morphology of breast cancer cells are examined under three-dimensional culture conditions. A microfluidic chip is then designed to create a three-dimensional environment with dimensions and geometry, as well as biological, chemical and mechanical conditions similar to the real microenvironmental conditions in the early stages of the breast tumor. The morphological changes, and behavior of breast cancer cells are studied within the three-dimensional cell cultures. Finally, the optimal conditions for the simultaneous culture of cancer and endothelial cells inside the chip will be examined so that in the future this culture can be performed and studied simultaneously under different conditions
  9. Keywords:
  10. Collagen ; Breast Cancer ; Microfluidic Chip ; Organ on Chip ; Three Dimentional Cell Culture ; Cancer Cells ; Endothelial Cell

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