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Effects of stabilizers on sustainability, activity and decolorization performance of Manganese Peroxidase enzyme produced by Phanerochaete chrysosporium

Emami, E ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1016/j.jece.2020.104459
  3. Publisher: Elsevier Ltd , 2020
  4. Abstract:
  5. Manganese Peroxidase (MnP), is one of the most promising lignin depolymerization enzymes, which has been widely used for degradation purposes. Nevertheless, MnP tends to lose activity rapidly during its maintenance phase and degradation process, especially in the inevitable presence of hydrogen peroxide. This study aimed to improve MnP efficiency produced by Phanerochaete chrysosporium, via enhancing its sustainability. In this context, the effects of MnO2, Fe3O4, PEG, Veratryl Alcohol (VA), and DMSO as stabilizing agents on MnP activity were explored both in vivo and in vitro. During in vivo experiments, heterogeneous Fe3O4 was found to be the desirable choice to enhance MnP production, obtaining a maximum MnP activity of 2149 U/L, which was a 67 % boost over the stock configuration. Also, in vitro studies demonstrated a noticeable increase in the stability of the extracted MnP from 8 days up to 14, 23, 23, 25, and 26 days by the addition of MnO2, VA, Fe3O4, PEG, and DMSO stabilizers, respectively. The efficiency of stabilizers on degradation ability of MnP was also examined; optimum conversion of model dyes viz., AO7 and CV, were improved 36.17 and 126.51 % in response to stabilizers, respectively. The inclusion of stabilizers managed to improve sustainability, performance, and cost-effectiveness of the biological water treatment process. © 2020 Elsevier Ltd
  6. Keywords:
  7. Biological process ; Decolorization ; Enzyme ; Manganese Peroxidase ; Phanerochaete chrysosporium ; Stabilizer ; Biological water treatment ; Cost effectiveness ; Dimethyl sulfoxide ; Efficiency ; Enzymes ; Magnetite ; Manganese oxide ; Biological water ; Degradation process ; In-vivo experiments ; Lignin depolymerization ; Stabilizing agents ; Veratryl alcohol ; Sustainable development ; Iron Oxides ; Manganese
  8. Source: Journal of Environmental Chemical Engineering ; Volume 8, Issue 6 , 2020
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S2213343720308083