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Designing a Ratiometric Probe for Naked Eye Detection of Hydrogen Peroxide

Mohammadpour, Fatemeh | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54150 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Hormozinezhad, Mohammad Reza
  7. Abstract:
  8. Hydrogen peroxide (H2O2) is of great importance in numerous fields such as pharmaceuticals, mining, textile, environmental and food industry. Therefore, the development of low cost, on-site, and uncomplicated H2O2 sensors are of high interest. To date, colloidal quantum dots (QDs) have been used to detect H2O2 based on the quenching of fluorescence intensity in a single wavelength. However, intensity of fluorescent signal could be easily disturbed by various factors. To overcome these undesirable effects, here, a ratiometric sensor has been developed by adding a second fluorophore (as reference) to QDs. For this purpose TGA-capped CdTe QDs were prepared. To detect H2O2, the ratiometric probe and phosphate buffer (pH 8.0) were transferred into a 3.0 mL microcentrifuge tube and were thoroughly mixed. Then, different concentration of H2O2 solution was added into the mixture, and the fluorescence spectra were recorded after 10 min incubation.The response of ratiometric probe to different concentration of H2O2 was monitored under the optimum conditions. The outcomes revealed that with increasing the concentration of H2O2, the fluorescence intensity of QDs was quenched dramatically, while the fluorescence intensity of the reference remained almost unchanged. The signal of (I0-I)/I0 was proportional toward H2O2 concentration from 12.0 μmol L-1 to 40.0 μmol L-1. A ratiometric fluorescent probe has been developed for naked-eye detection of H2O2. The proposed probe has shown no interruption from the co-exciting compounds and allowed for determination of H2O2 in urine samples. Compared to previous methods, our newly developed sensor possesses the advantages of simplicity, low cost, and providing the visual detection
  9. Keywords:
  10. Naked Eye Detection ; Hydrogen Peroxide ; Carbon Dots ; Quantum Dots ; Fluorescence Nanostructures ; Fluorescence Ratiometric ; Fluorescence Sensors

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