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Design, Expression, and Evaluation of Nanoparticle Structure Containing Malaria Vaccine Candidate Antigen by Self-assembled Peptide Nanoparticles (SAPNs) and Confirmation of its Antigenicity Using Serum of Malaria Patients

Zahedi, Farhad | 2021

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54273 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran; Abouei Mehrizi, Akram; Sardari, Soroush; Zakeri, Sedigheh
  7. Abstract:
  8. Malaria is one of the most important infectious diseases in the tropical and subtropical regions of the world and is caused in humans by the bite of the female Anopheles mosquito of five species of Plasmodium. According to the World Health Organization, about 229 million people worldwide were infected in 2019 by the disease, and 409,000 people died from the disease. Resistance of the parasite against drugs and resistance of vectors to insecticides and larvicides, which prevents the elimination and eradication of malaria, shows the need for new strategies, such as having an effective vaccine against this disease. In the second generation of vaccines, to develop malaria vaccines, recombinant proteins are used. Unfortunately, recombinant proteins are weak immunogens. One way to increase the immunogenicity of proteins is to convert the peptides to nanoparticles by aggregating them together to form nanoscale particles called self-assembled peptide nanoparticles (SAPNs). In this project, a SAPN based on two HAP2-GCS and cd loop domains of Plasmodium falciparum GCS1 antigen was designed and validated in silico by bioinformatics methods and molecular dynamics simulations. The synthetic gene was synthesized (according to the design), cloned, and expressed in the prokaryotic expression system. SAPN size was confirmed in nano dimensions by the DLS test. The shape of the SAPN was spherical with the approval of FESEM and TEM. SAPN antigenicity was approved using malaria patients' serum by Western blot and ELISA tests
  9. Keywords:
  10. Malaria Diease ; Molecular Dynamic Simulation ; Recombinant Protein ; Malaria Vaccines ; Self Assembled Peptide Nanoparticle (SAPN) ; Nanovaccine

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