Loading...
Search for: nonhuman
0.013 seconds
Total 314 records

    Evaluation of the effects of fullerene c60 nanoparticles on oxidative stress parameters in normal rats liver and brain

    , Article Journal of Advances in Medical and Biomedical Research ; Volume 27, Issue 124 , 2019 , Pages 8-15 ; 26766264 (ISSN) Namadr, F ; Bahrami, F ; Bahri, Z ; Ghanbari, B ; Elahi, S. A ; Mohammadi, M. T ; Sharif University of Technology
    Zanjan University of Medical Sciences and Health Services  2019
    Abstract
    Background & Objective: The potent antioxidant property of fullerene C60 nanoparticles and their derivatives has been demonstrated in a wide range of in vitro and in vivo studies. Hence, we examined the effects of fullerene C60 on the oxidative stress parameters in brain and liver of the rats in normal situation. Materials & Methods: The study was performed in two groups of Wistar rats (each group, n = 6); normal and fullerene-treated normal animals. Treated rats received fullerene via oral gavage at dose of 1 mg/kg/day for 60 days. At termination of the study, the oxidative stress parameters were determined in brain and liver tissues, including the contents of glutathione (GSH) and... 

    A novel pattern matching algorithm for genomic patterns related to protein motifs

    , Article Journal of Bioinformatics and Computational Biology ; Volume 18, Issue 1 , 2020 Foroughmand Araabi, M. H ; Goliaei, S ; Goliaei, B ; Sharif University of Technology
    World Scientific Publishing Co. Pte Ltd  2020
    Abstract
    Patterns on proteins and genomic sequences are vastly analyzed, extracted and collected in databases. Although protein patterns originate from genomic coding regions, very few works have directly or indirectly dealt with coding region patterns induced from protein patterns. Results: In this paper, we have defined a new genomic pattern structure suitable for representing induced patterns from proteins. The provided pattern structure, which is called "Consecutive Positions Scoring Matrix (CPSSM)", is a replacement for protein patterns and profiles in the genomic context. CPSSMs can be identified, discovered, and searched in genomes. Then, we have presented a novel pattern matching algorithm... 

    Comparison of strengths of five internal fixation methods used after bilateral sagittal split ramus osteotomy: An in vitro study

    , Article Dental Research Journal ; Volume 17, Issue 4 , 2020 , Pages 258-265 Sarkarat, F ; Ahmady, A ; Farahmand, F ; Fateh, A ; Kahali, R ; Nourani, A ; Rakhshan, V ; Sharif University of Technology
    Wolters Kluwer Medknow Publications  2020
    Abstract
    Results on the strength and displacement of internal fixation methods for bilateral sagittal split ramus osteotomy are controversial, and some designs have not been adequately studied. Therefore, this study was conducted to compare techniques using bicortical or monocortical screws. Materials and Methods: In this in vitro study, 35 sheep hemi-mandibles were randomly assigned to five groups of seven each: fixation using (1) a 13 × 2 screw, (2) two 13 × 2 screws (arranged vertically), (3) three 13 × 2 screws, (4) 1 plate with 4 holes and four monocortical screws, and (5) a Y-shaped plate and five monocortical screws. Specimens underwent vertical forces until failure. Breakage forces and... 

    Advances in skin regeneration: application of electrospun scaffolds

    , Article Advanced Healthcare Materials ; Volume 4, Issue 8 , 2015 , Pages 1114-1133 ; 21922640 (ISSN) Norouzi, M ; Boroujeni, S. M ; Omidvarkordshouli, N ; Soleimani, M ; Sharif University of Technology
    Wiley-VCH Verlag  2015
    Abstract
    The paucity of cellular and molecular signals essential for normal wound healing makes severe dermatological ulcers stubborn to heal. The novel strategies of skin regenerative treatments are focused on the development of biologically responsive scaffolds accompanied by cells and multiple biomolecules resembling structural and biochemical cues of the natural extracellular matrix (ECM). Electrospun nanofibrous scaffolds provide similar architecture to the ECM leading to enhancement of cell adhesion, proliferation, migration and neo tissue formation. This Review surveys the application of biocompatible natural, synthetic and composite polymers to fabricate electrospun scaffolds as skin... 

    Chemometrics comparison of gas chromatography with mass spectrometry and comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry Daphnia magna metabolic profiles exposed to salinity

    , Article Journal of Separation Science ; Volume 41, Issue 11 , 2018 , Pages 2368-2379 ; 16159306 (ISSN) Parastar, H ; Garreta Lara, E ; Campos, B ; Barata, C ; Lacorte, S ; Tauler, R ; Sharif University of Technology
    Wiley-VCH Verlag  2018
    Abstract
    The performances of gas chromatography with mass spectrometry and of comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry are examined through the comparison of Daphnia magna metabolic profiles. Gas chromatography with mass spectrometry and comprehensive two-dimensional gas chromatography with mass spectrometry were used to compare the concentration changes of metabolites under saline conditions. In this regard, a chemometric strategy based on wavelet compression and multivariate curve resolution–alternating least squares is used to compare the performances of gas chromatography with mass spectrometry and comprehensive two-dimensional gas chromatography with... 

    A continuous flow microfluidic device based on contactless dielectrophoresis for bioparticles enrichment

    , Article Electrophoresis ; Volume 39, Issue 3 , 2018 , Pages 445-455 ; 01730835 (ISSN) Rahmani, A ; Mohammadi, A. A ; Kalhor, H. R ; Sharif University of Technology
    Wiley-VCH Verlag  2018
    Abstract
    In recent years, applications of dielectrophoresis-based platforms have been recognized as effective and dependable approach to separate cells and bioparticles, suspended in different carrier fluids, based on particle size and electrical properties. In this study, a microfluidic device was fabricated by an unprecedented electrode pattern, and several experiments were performed to enrich samples including either of yeast, Escherichia coli, or latex particles. A chemical deposition-based method was employed for fabrication of microelectrodes, inducing nonuniform electric field required for dielectrophoresis-based separation. One major advantage of our employed method is low fabrication cost,... 

    Controlling differentiation of stem cells for developing personalized organ-on-chip platforms

    , Article Advanced Healthcare Materials ; Volume 7, Issue 2 , 2018 ; 21922640 (ISSN) Geraili, A ; Jafari, P ; Sheikh Hassani, M ; Heidary Araghi, B ; Mohammadi, M. H ; Ghafari, A. M ; Hassanpour Tamrin, S ; Pezeshgi Modarres, H ; Rezaei Kolahchi, A ; Ahadian, S ; Sanati Nezhad, A ; Sharif University of Technology
    Wiley-VCH Verlag  2018
    Abstract
    Organ-on-chip (OOC) platforms have attracted attentions of pharmaceutical companies as powerful tools for screening of existing drugs and development of new drug candidates. OOCs have primarily used human cell lines or primary cells to develop biomimetic tissue models. However, the ability of human stem cells in unlimited self-renewal and differentiation into multiple lineages has made them attractive for OOCs. The microfluidic technology has enabled precise control of stem cell differentiation using soluble factors, biophysical cues, and electromagnetic signals. This study discusses different tissue- and organ-on-chip platforms (i.e., skin, brain, blood–brain barrier, bone marrow, heart,... 

    Improvement of size-based particle separation throughput in slanted spiral microchannel by modifying outlet geometry

    , Article Electrophoresis ; Volume 41, Issue 5-6 , February , 2020 , Pages 353-359 Mihandoust, A ; Maleki Jirsaraei, N ; Rouhani, S ; Safi, S ; Alizadeh, M ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Abstract
    The inertial microfluidic technique, as a powerful new tool for accurate cell/particle separation based on the hydrodynamic phenomenon, has drawn considerable interest in recent years. Despite numerous microfluidic techniques of particle separation, there are few articles in the literature on separation techniques addressing external outlet geometry to increase the throughput efficiency and purity. In this work, we report on a spiral inertial microfluidic device with high efficiency (>98%). Herein, we demonstrate how changing the outlet geometry can improve the particle separation throughput. We present a complete separation of 4 and 6 μm from 10 μm particles potentially applicable to... 

    Temporal activation of LRH-1 and RAR-γ in human pluripotent stem cells induces a functional naïve-like state

    , Article EMBO Reports ; Volume 21, Issue 10 , 2020 Taei, A ; Kiani, T ; Taghizadeh, Z ; Moradi, S ; Samadian, A ; Mollamohammadi, S ; Sharifi Zarchi, A ; Guenther, S ; Akhlaghpour, A ; Asgari Abibeiglou, B ; Najar Asl, M ; Karamzadeh, R ; Khalooghi, K ; Braun, T ; Hassani, S. N ; Baharvand, H ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Abstract
    Naïve pluripotency can be established in human pluripotent stem cells (hPSCs) by manipulation of transcription factors, signaling pathways, or a combination thereof. However, differences exist in the molecular and functional properties of naïve hPSCs generated by different protocols, which include varying similarities with pre-implantation human embryos, differentiation potential, and maintenance of genomic integrity. We show here that short treatment with two chemical agonists (2a) of nuclear receptors, liver receptor homologue-1 (LRH-1) and retinoic acid receptor gamma (RAR-γ), along with 2i/LIF (2a2iL) induces naïve-like pluripotency in human cells during reprogramming of fibroblasts,... 

    A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration

    , Article Advanced Healthcare Materials ; Volume 10, Issue 3 , 2021 ; 21922640 (ISSN) Ahmadian, Z ; Correia, A ; Hasany, M ; Figueiredo, P ; Dobakhti, F ; Eskandari, M. R ; Hosseini, S.H ; Abiri, R ; Khorshid, S ; Hirvonen, J ; Santos, H. A ; Shahbazi, M. A ; Sharif University of Technology
    Wiley-VCH Verlag  2021
    Abstract
    Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs,... 

    A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration

    , Article Advanced Healthcare Materials ; Volume 10, Issue 3 , 2021 ; 21922640 (ISSN) Ahmadian, Z ; Correia, A ; Hasany, M ; Figueiredo, P ; Dobakhti, F ; Eskandari, M. R ; Hosseini, S. H ; Abiri, R ; Khorshid, S ; Hirvonen, J ; Santos, H. A ; Shahbazi, M. A ; Sharif University of Technology
    Wiley-VCH Verlag  2021
    Abstract
    Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs,... 

    Microwave-assisted extraction and high-throughput monolithic-polymer-based micro-solid-phase extraction of organophosphorus, triazole, and organochlorine residues in apple

    , Article Journal of Separation Science ; Volume 39, Issue 3 , 2016 , Pages 576-583 ; 16159306 (ISSN) Bagheri, H ; Es Haghi, A ; Basiri Pour, F ; Sharif University of Technology
    Wiley-VCH Verlag 
    Abstract
    A high-throughput micro-solid-phase extraction device based on a 96-well plate was constructed and applied to the determination of pesticide residues in various apple samples. Butyl methacrylate and ethylene glycol dimethacrylate were copolymerized as a monolithic polymer and placed in the cylindrically shaped stainless-steel meshes of 96-micro-solid-phase extraction device and used as an extracting unit. Before the micro-solid-phase extraction, microwave-assisted extraction was employed to facilitate the transfer of the pesticide residues from the apple matrix to liquid media. Then, 1 mL of the aquatic samples was transferred into the 96-well plate and the 96-micro-solid-phase extraction... 

    Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model

    , Article Journal of Cellular Physiology ; Volume 234, Issue 11 , 2019 , Pages 20742-20754 ; 00219541 (ISSN) Mohammadshirazi, A ; Sadrosadat, H ; Jaberi, R ; Zareikheirabadi, M ; Mirsadeghi, S ; Naghdabadi, Z ; Ghaneezabadi, M ; Fardmanesh, M ; Baharvand, H ; Kiani, S ; Sharif University of Technology
    Wiley-Liss Inc  2019
    Abstract
    A large number of treatment approaches have been used for spinal cord injury improvement, a medically incurable disorder, and subsequently stem cell transplantation appears to be a promising strategy. The main objective of this study is to ascertain whether combinational therapy of human neural stem cells (hNSCs) together with lithium chloride improves cell survival, proliferation, and differentiation in a rat spinal contusion model, or not. Contusive spinal cord injury was implemented on Wistar male rats. Experimental groups comprised of: control, hNSCs transplanted, lithium chloride (Li), and hNSCs and lithium chloride (hNSCs + Li). In every experimental group, locomotor activity score and... 

    PH-Sensitive stimulus-responsive nanocarriers for targeted delivery of therapeutic agents

    , Article Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology ; Volume 8, Issue 5 , 2016 , Pages 696-716 ; 19395116 (ISSN) Karimi, M ; Eslami, M ; Sahandi Zangabad, P ; Mirab, F ; Farajisafiloo, N ; Shafaei, Z ; Ghosh, D ; Bozorgomid, M ; Dashkhaneh, F ; Hamblin, M. R ; Sharif University of Technology
    Wiley-Blackwell  2016
    Abstract
    In recent years miscellaneous smart micro/nanosystems that respond to various exogenous/endogenous stimuli including temperature, magnetic/electric field, mechanical force, ultrasound/light irradiation, redox potentials, and biomolecule concentration have been developed for targeted delivery and release of encapsulated therapeutic agents such as drugs, genes, proteins, and metal ions specifically at their required site of action. Owing to physiological differences between malignant and normal cells, or between tumors and normal tissues, pH-sensitive nanosystems represent promising smart delivery vehicles for transport and delivery of anticancer agents. Furthermore, pH-sensitive systems... 

    Accuracy Quantification of the Reverse Engineering and High-Order Finite Element Analysis of Equine MC3 Forelimb

    , Article Journal of Equine Veterinary Science ; Volume 78 , 2019 , Pages 94-106 ; 07370806 (ISSN) Mouloodi, S ; Rahmanpanah, H ; Burvill, C ; Davies, H. M. S ; Sharif University of Technology
    W.B. Saunders  2019
    Abstract
    Shape is a key factor in influencing mechanical responses of bones. Considered to be smart viscoelastic and inhomogeneous materials, bones are stimulated to change shape (model and remodel) when they experience changes in the compressive strain distribution. Using reverse engineering techniques via computer-aided design (CAD) is crucial to create a virtual environment to investigate the significance of shape in biomechanical engineering. Nonetheless, data are lacking to quantify the accuracy of generated models and to address errors in finite element analysis (FEA). In the present study, reverse engineering through extrapolating cross-sectional slices was used to reconstruct the diaphysis of... 

    Label-Free real-time detection of HBsAg using a QCM immunosensor

    , Article Clinical Laboratory ; Volume 68, Issue 4 , 2022 , Pages 707-720 ; 14336510 (ISSN) Saffari, Z ; Ghavidel, A ; Ahangari Cohan, R ; Hamidi Fard, M ; Khoobi, M ; Aghasadeghi, M ; Norouzian, D ; Sharif University of Technology
    Verlag Klinisches Labor GmbH  2022
    Abstract
    Background: Hepatitis B virus surface antigen (HBsAg) is an important protein in both diagnosis and prevention of hepatitis B infection. In the current study, a piezoelectric immunosensor based on antibody-antigen interaction was designed to detect HBsAg. A quartz crystal microbalance system was employed to detect antibody-antigen interaction. Methods: At first, an oscillator was designed to measure the resonant frequency affected by the reactants using IC 74LVC1GX04. Antibody against HBsAg was immobilized on 10 MHz AT-cut quartz crystal. The surface modifications were monitored by atomic force microscopy (AFM) and contact angle measurements. Different concentrations of antibody were used... 

    Comparing the effects of endurance and resistance trainings on gene expression involved in protein synthesis and degradation signaling pathways of Wistar rat soleus muscle

    , Article Tehran University Medical Journal ; Volume 77, Issue 11 , 2020 , Pages 668-677 Gholipour, M ; Seifabad, M ; Asad, M. R ; Sharif University of Technology
    Tehran University of Medical Sciences  2020
    Abstract
    Background: Skeletal muscle mass, which is regulated by a balance between muscle protein synthesis and degradation, is an important factor for movement to meet everyday needs, especially in pathological conditions and aging. The purpose of the present investigation was to compare the alterations of the gene expression involved in muscle protein synthesis and degradation signaling pathways induced by two exercise training protocols. Methods: Eight weeks old Wistar rats have been assigned to the present experimental study, which was conducted from August 2018 to October 2018 at the animal laboratory of Tehran University. They were randomly divided into two resistance and endurance training... 

    The effect of temperature on eccentric contraction-induced isometric force loss in isolated perfused rat medial gastrocnemius muscle

    , Article Tehran University Medical Journal ; Volume 66, Issue 6 , 2008 , Pages 388-395 ; 16831764 (ISSN) Vasaghi Gharamaleki, B ; Keshavarz, M ; Gharibzadeh, S ; Marvi, H ; Mosayebnejad, J ; Ebrahimi Takamjani, E ; Sharif University of Technology
    Tehran University of Medical Sciences  2008
    Abstract
    Background: The typical features of eccentric exercise-induced muscle damage are delayed-onset muscle soreness (DOMS) and prolonged loss of muscle strength. It has been shown that passive warmth is effective in reducing muscle injury. Due to the interaction of different systems in vivo, we used isolated perfused medial gastrocnemius skeletal muscle to study the direct effect of temperature on the eccentric contraction-induced force loss. Methods: After femoral artery cannulation of a rat, the left medial gastrocnemius muscle was separated and then the entire lower limb was transferred into a prewarmed (35oC) chamber. With the chamber temperature at 31, 35 and 39oC before and during eccentric... 

    SAR thresholds for electromagnetic exposure using functional thermal dose limits

    , Article International Journal of Hyperthermia ; Volume 34, Issue 8 , 2018 , Pages 1248-1254 ; 02656736 (ISSN) Adibzadeh, F ; Paulides, M. M ; Van Rhoon, G. C ; Sharif University of Technology
    Taylor and Francis Ltd  2018
    Abstract
    Background and purpose: To protect against any potential adverse effects to human health from localised exposure to radio frequency (100 kHz–3 GHz) electromagnetic fields (RF EMF), international health organisations have defined basic restrictions on specific absorption rate (SAR) in tissues. These exposure restrictions incorporate safety factors which are generally conservative so that exposures that exceed the basic restrictions are not necessarily harmful. The magnitude of safety margin for various exposure scenarios is unknown. This shortcoming becomes more critical for medical applications where the safety guidelines are required to be relaxed. The purpose of this study was to quantify... 

    Plant protein-based hydrophobic fine and ultrafine carrier particles in drug delivery systems

    , Article Critical Reviews in Biotechnology ; Volume 38, Issue 1 , 2018 , Pages 47-67 ; 07388551 (ISSN) Malekzad, H ; Mirshekari, H ; Sahandi Zangabad, P ; Moosavi Basri, S. M ; Baniasadi, F ; Sharifi Aghdam, M ; Karimi, M ; Hamblin, M. R ; Sharif University of Technology
    Taylor and Francis Ltd  2018
    Abstract
    For thousands of years, plants and their products have been used as the mainstay of medicinal therapy. In recent years, besides attempts to isolate the active ingredients of medicinal plants, other new applications of plant products, such as their use to prepare drug delivery vehicles, have been discovered. Nanobiotechnology is a branch of pharmacology that can provide new approaches for drug delivery by the preparation of biocompatible carrier nanoparticles (NPs). In this article, we review recent studies with four important plant proteins that have been used as carriers for targeted delivery of drugs and genes. Zein is a water-insoluble protein from maize; Gliadin is a 70% alcohol-soluble...