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    کلیدواژه های تکراریCurcumin loading potentiates the neuroprotective efficacy of Fe3O4 magnetic nanoparticles in cerebellum cells of schizophrenic rats

    , Article Biomedicine and Pharmacotherapy ; Volume 108 , 2018 , Pages 1244-1252 ; 07533322 (ISSN) Naserzadeh, P ; Ashrafi Hafez, A ; Abdorahim, M ; Abdollahifar, M. A ; Shabani, R ; Peirovi, H ; Simchi, A ; Ashtari, K ; Sharif University of Technology
    Background: The aim of this study was to investigate the neurotoxic effects of Fe3O4 magnetic- CurNPs on isolated schizophrenia mitochondria of rats as an in vivo model. Methods: We designed CMN loaded superparamagnetic iron oxide nanoparticles (SPIONs) (Fe3O4 magnetic- CurNPs) to achieve an enhanced therapeutic effect. The physicochemical properties of Fe3O4 magnetic- CurNPs were characterized using X-ray diffraction (XRD), and dynamic laser light scattering (DLS) and zeta potential. Further, to prove Fe3O4 magnetic- CurNPs results in superior therapeutic effects, and also, the mitochondrial membrane potential collapse, mitochondrial complex II activity, reactive oxygen species generation,... 

    You are what you eat: Sequence analysis reveals how plant microRNAs may regulate the human genome

    , Article Computers in Biology and Medicine ; Volume 106 , 2019 , Pages 106-113 ; 00104825 (ISSN) Kashani, B ; Hasani Bidgoli, M ; Motahari, S. A ; Sedaghat, N ; Modarressi, M. H ; Sharif University of Technology
    Elsevier Ltd  2019
    Background: Nutrigenomic has revolutionized our understanding of nutrition. As plants make up a noticeable part of our diet, in the present study we chose microRNAs of edible plants and investigated if they can perfectly match human genes, indicating potential regulatory functionalities. Methods: miRNAs were obtained using the PNRD database. Edible plants were separated and microRNAs in common in at least four of them entered our analysis. Using vmatchPattern, these 64 miRNAs went through four steps of refinement to improve target prediction: Alignment with the whole genome (2581 results), filtered for those in gene regions (1371 results), filtered for exon regions (66 results) and finally... 

    Whole genome sequencing of SARS-CoV2 strains circulating in Iran during five waves of pandemic

    , Article PLoS ONE ; Volume 17, Issue 5 May , 2022 ; 19326203 (ISSN) Yavarian, J ; Nejati, A ; Salimi, V ; Jandaghi, N.Z.S ; Sadeghi, K ; Abedi, A ; Zarchi, A. S ; Gouya, M. M ; Mokhtari Azad, T ; Sharif University of Technology
    Public Library of Science  2022
    Purpose Whole genome sequencing of SARS-CoV2 is important to find useful information about the viral lineages, variants of interests and variants of concern. As there are not enough data about the circulating SARS-CoV2 variants in Iran, we sequenced 54 SARS-CoV2 genomes during the 5 waves of pandemic in Iran. Methods After viral RNA extraction from clinical samples collected during the COVID-19 pandemic, next generation sequencing was performed using the Nextseq platform. The sequencing data were analyzed and compared with reference sequences. Results During the 1st wave, V and L clades were detected. The second wave was recognized by G, GH and GR clades. Circulating clades during the 3rd... 

    WalkIm: Compact image-based encoding for high-performance classification of biological sequences using simple tuning-free CNNs

    , Article PLoS ONE ; Volume 17, Issue 4 April , 2022 ; 19326203 (ISSN) Akbari Rokn Abadi, S ; Mohammadi, A ; Koohi, S ; Sharif University of Technology
    Public Library of Science  2022
    The classification of biological sequences is an open issue for a variety of data sets, such as viral and metagenomics sequences. Therefore, many studies utilize neural network tools, as the well-known methods in this field, and focus on designing customized network structures. However, a few works focus on more effective factors, such as input encoding method or implementation technology, to address accuracy and efficiency issues in this area. Therefore, in this work, we propose an image-based encoding method, called as WalkIm, whose adoption, even in a simple neural network, provides competitive accuracy and superior efficiency, compared to the existing classification methods (e.g. VGDC,... 

    Vis-NIR hyperspectral imaging coupled with independent component analysis for saffron authentication

    , Article Food Chemistry ; Volume 393 , 2022 ; 03088146 (ISSN) Hashemi Nasab, F. S ; Parastar, H ; Sharif University of Technology
    Elsevier Ltd  2022
    In the present contribution, visible-near infrared hyperspectral imaging (Vis-NIR-HSI) combined with a novel chemometric approach based on mean-filed independent component analysis (MF-ICA) followed by multivariate classification techniques is proposed for saffron authentication and adulteration detection. First, MF-ICA was used to exploit pure spatial and spectral profiles of the components. Then, principal component analysis (PCA) and hierarchical cluster analysis (HCA) were used to find patterns of authentic samples based on their distribution maps. Then, detection of five common plant-derived adulterants of saffron including safflower, saffron style, calendula, rubia and turmeric were... 

    Virotheranostics, a double-barreled viral gun pointed toward cancer; Ready to shoot?

    , Article Cancer Cell International ; Volume 20, Issue 1 , 2020 Keshavarz, M ; Sabbaghi, A ; Miri, S. M ; Rezaeyan, A ; Arjeini, Y ; Ghaemi, A ; Sharif University of Technology
    BioMed Central Ltd  2020
    Compared with conventional cancer treatments, the main advantage of oncolytic virotherapy is its tumor-selective replication followed by the destruction of malignant cells without damaging healthy cells. Accordingly, this kind of biological therapy can potentially be used as a promising approach in the field of cancer management. Given the failure of traditional monitoring strategies (such as immunohistochemical analysis (in providing sufficient safety and efficacy necessary for virotherapy and continual pharmacologic monitoring to track pharmacokinetics in real-time, the development of alternative strategies for ongoing monitoring of oncolytic treatment in a live animal model seems... 

    Use of gas chromatography-mass spectrometry combined with resolution methods to characterize the essential oil components of Iranian cumin and caraway

    , Article Journal of Chromatography A ; Volume 1143, Issue 1-2 , 2007 , Pages 215-226 ; 00219673 (ISSN) Jalali Heravi, M ; Zekavat, B ; Sereshti, H ; Sharif University of Technology
    Gas chromatography-mass spectrometry combined with iterative and non-iterative resolution methods was used to characterize the essential oil components of Iranian cumin and caraway. Orthogonal projection resolution (OPR) as a non-iterative and distance-selection-multivariate curve resolution-alternative least squares (DS-MCR-ALS) as an iterative method were used as auxiliary means to the analysis in the case of overlapping peaks. A total of 19 and 39 components were identified by direct similarity searches for cumin and caraway oils, respectively. These numbers were extended to 49 and 98 components, respectively with the help of chemometric techniques. Major constituents in cumin are... 

    Upgrading activated sludge systems and reduction in excess sludge

    , Article Bioresource Technology ; Volume 102, Issue 22 , November , 2011 , Pages 10327-10333 ; 09608524 (ISSN) Hazrati, H ; Shayegan, J ; Sharif University of Technology
    Most of 200 Activated Sludge Plant in Iran are overloaded and as a result, their efficiency is low. In this work, a pilot plant is manufactured and put into operation in one of the wastewater treatment plants in the west of Tehran. Instead of conventional activated sludge, a membrane bioreactor and an upflow anaerobic sludge blanket reactor used as a pretreatment unit in this pilot. For the sake of data accuracy and precision, an enriched municipal wastewater was opted as an influent to the pilot. Based on the attained result, the optimum retention time in this system was 4. h, and the overall COD removal efficiency was 98%. As a whole, the application of this retrofit would increase the... 

    Unraveling cancer metastatic cascade using microfluidics-based technologies

    , Article Biophysical Reviews ; Volume 14, Issue 2 , 2022 , Pages 517-543 ; 18672450 (ISSN) Hakim, M ; Kermanshah, L ; Abouali, H ; Hashemi, H. M ; Yari, A ; Khorasheh, F ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
    Springer Science and Business Media Deutschland GmbH  2022
    Cancer has long been a leading cause of death. The primary tumor, however, is not the main cause of death in more than 90% of cases. It is the complex process of metastasis that makes cancer deadly. The invasion metastasis cascade is the multi-step biological process of cancer cell dissemination to distant organ sites and adaptation to the new microenvironment site. Unraveling the metastasis process can provide great insight into cancer death prevention or even treatment. Microfluidics is a promising platform, that provides a wide range of applications in metastasis-related investigations. Cell culture microfluidic technologies for in vitro modeling of cancer tissues with fluid flow and the... 

    Type V collagen in scar tissue regulates the size of scar after heart injury

    , Article Cell ; Volume 182, Issue 3 , 2020 , Pages 545-562.e23 Yokota, T ; McCourt, J ; Ma, F ; Ren, S ; Li, S ; Kim, T. H ; Kurmangaliyev, Y. Z ; Nasiri, R ; Ahadian, S ; Nguyen, T ; Tan, X. H. M ; Zhou, Y ; Wu, R ; Rodriguez, A ; Cohn, W ; Wang, Y ; Whitelegge, J ; Ryazantsev, S ; Khademhosseini, A ; Teitell, M. A ; Chiou, P. Y ; Birk, D. E ; Rowat, A. C ; Crosbie, R. H ; Pellegrini, M ; Seldin, M ; Lusis, A. J ; Deb, A ; Sharif University of Technology
    Cell Press  2020
    Scar tissue size following myocardial infarction is an independent predictor of cardiovascular outcomes, yet little is known about factors regulating scar size. We demonstrate that collagen V, a minor constituent of heart scars, regulates the size of heart scars after ischemic injury. Depletion of collagen V led to a paradoxical increase in post-infarction scar size with worsening of heart function. A systems genetics approach across 100 in-bred strains of mice demonstrated that collagen V is a critical driver of postinjury heart function. We show that collagen V deficiency alters the mechanical properties of scar tissue, and altered reciprocal feedback between matrix and cells induces... 

    Two schemes for production of biosurfactant from Pseudomonas aeruginosa MR01: Applying residues from soybean oil industry and silica sol–gel immobilized cells

    , Article Colloids and Surfaces B: Biointerfaces ; Volume 152 , 2017 , Pages 159-168 ; 09277765 (ISSN) Bagheri Lotfabad, T ; Ebadipour, N ; Roostaazad, R ; Partovi, M ; Bahmaei, M ; Sharif University of Technology
    Elsevier B.V  2017
    Rhamnolipids are the most common biosurfactants and P. aeruginosa strains are the most frequently studied microorganisms for the production of rhamnolipids. Eco-friendly advantages and promising applications of rhamnolipids in various industries are the major reasons for pursuing the economic production of these biosurfactants. This study shows that cultivation of P. aeruginosa MR01 in medium contained inexpensive soybean oil refinery wastes which exhibited similar levels and homologues of rhamnolipids. Mass spectrometry indicated that the Rha-C10-C10 and Rha-Rha-C10-C10 constitute the main rhamnolipids in different cultures of MR01 including one of oil carbon source analogues. Moreover,... 

    Two-compartment processing as a tool to boost recombinant protein production

    , Article Engineering in Life Sciences ; Vol. 14, issue. 2 , March , 2014 , p. 118-128 Jazini, M ; Herwig, C ; Sharif University of Technology
    Pichia pastoris is used extensively as a production platform for many recombinant proteins. The dissolved oxygen (DO) is one of the most important factors influencing protein production. The influence of the DO on productivity has not been studied independent from the feed rate. In this work, various DO levels were investigated independent from the feed rate. The model system was recombinant P. pastoris under the control of methanol-induced alcohol oxidase promoter, which expressed HRP as the target protein. No significant effect was observed in terms of titer and specific productivity, which is a confirmation of the fact that the DO in a one-compartment system cannot boost productivity for... 

    Tissue growth into three-dimensional composite scaffolds with controlled micro-features and nanotopographical surfaces

    , Article Journal of Biomedical Materials Research - Part A ; Volume 101, Issue 10 , 2013 , Pages 2796-2807 ; 15493296 (ISSN) Tamjid, E ; Simchi, A ; Dunlop, J. W. C ; Fratzl, P ; Bagheri, R ; Vossoughi, M ; Sharif University of Technology
    Controlling topographic features at all length scales is of great importance for the interaction of cells with tissue regenerative materials. We utilized an indirect three-dimensional printing method to fabricate polymeric scaffolds with pre-defined and controlled external and internal architecture that had an interconnected structure with macro- (400-500 μm) and micro- (∼25 μm) porosity. Polycaprolactone (PCL) was used as model system to study the kinetics of tissue growth within porous scaffolds. The surface of the scaffolds was decorated with TiO2 and bioactive glass (BG) nanoparticles to the better match to nanoarchitecture of extracellular matrix (ECM). Micrometric BG particles were... 

    Three-dimensional simulation of urine concentrating mechanism in a functional unit of rat outer medulla. I. Model structure and base case results

    , Article Mathematical Biosciences ; Vol. 258 , 2014 , pp. 44-56 ; ISSN: 00255564 Sohrabi, S ; Saidi, M. S ; Saadatmand, M ; Banazadeh, M. H ; Firoozabadi, B ; Sharif University of Technology
    The urine formation and excretion system have long been of interest for mathematicians and physiologists to elucidate the obscurities within the process happens in renal tissue. In this study, a novel three-dimensional approach is utilized for modeling the urine concentrating mechanism in rat renal outer medulla which is essentially focused on demonstrating the significance of tubule's architecture revealed in anatomic studies and physiological literature. Since nephrons and vasculatures work interdependently through a highly structured arrangement in outer medulla which is dominated by vascular bundles, a detailed functional unit is proposed based on this specific configuration.... 

    The synergic effects of anionic and cationic chemical surfactants, and bacterial solution on wettability alteration of carbonate rock: an experimental investigation

    , Article Colloids and Surfaces A: Physicochemical and Engineering Aspects ; Volume 513 , 2017 , Pages 422-429 ; 09277757 (ISSN) Hajibagheri, F ; Lashkarbolooki, M ; Ayatollahi, S ; Hashemi, A ; Sharif University of Technology
    Changing the wettability of reservoir rock towards strongly water-wet state is effective way to enhance oil recovery from fractured carbonate reservoirs which are typically oil-wet. Regarding this fact, the injection of surfactant and the bacterial solution as EOR agents is proposed in the current work as a potential method to alter the wettability of rock surface reservoir. Nevertheless, there is a definite lack of experimental data regarding this method and the synergistic effect of both chemical and bacterial solutions on this process. In this study, the sole and combined effects of the bacterial solution using an Enterobacter cloacae strain as a biosurfactant-producer are compared with... 

    The role of the gut microbiota and nutrition on spatial learning and spatial memory: a mini review based on animal studies

    , Article Molecular Biology Reports ; Volume 49, Issue 2 , 2022 , Pages 1551-1563 ; 03014851 (ISSN) Alemohammad, S.M.A ; Noori, S. M. R ; Samarbafzadeh, E ; Noori, S. M. A ; Sharif University of Technology
    Springer Science and Business Media B.V  2022
    The gut-brain axis is believed to constitute a bidirectional communication mechanism that affects both mental and digestive processes. Recently, the role of the gut microbiota in cognitive performance has been the focus of much research. In this paper, we discuss the effects of gut microbiota and nutrition on spatial memory and learning. Studies have shown the influence of diet on cognitive capabilities such as spatial learning and memory. It has been reported that a high-fat diet can alter gut microbiota which subsequently leads to changes in spatial learning and memory. Some microorganisms in the gut that can significantly affect spatial learning and memory are Akkermansia muciniphila,... 

    The role of hippo signaling pathway in physiological cardiac hypertrophy

    , Article BioImpacts ; Volume 10, Issue 4 , 2020 , Pages 251-257 Gholipour, M ; Tabrizi, A ; Sharif University of Technology
    Tabriz University of Medical Sciences  2020
    Introduction: The role of Hippo signaling pathway, which was identified by genetic studies as a key regulator for tissue growth and organ size, in promoting physiological cardiac hypertrophy has not been investigated. Methods: Fourteen male Wistar rats were randomly assigned to the exercise and control groups. The exercise group ran 1 hour per day, 5 days/week, at about 65%-75% VO2max on the motor-driven treadmill with 15ºslope, and the control group ran 15 min/d, 2 days/ week at 9 m/min (0ºinclination), throughout the eight-week experimental period. Forty-eight hours after the last session, hearts were dissected and left ventricles were weighed and stored for subsequent RT-PCR analysis.... 

    The role of co-solvents in improving the direct transesterification of wet microalgal biomass under supercritical condition

    , Article Bioresource Technology ; Volume 193 , October , 2015 , Pages 90-96 ; 09608524 (ISSN) Abedini Najafabadi, H ; Vossoughi, M ; Pazuki, G ; Sharif University of Technology
    Elsevier Ltd  2015
    In this research, direct conversion of wet algal biomass into biodiesel using supercritical methanol was studied. In this process, microalgal lipids simultaneously was extracted and converted to biodiesel under high pressure and temperature conditions without using any catalyst. Several experiments have been performed to optimize the methanol amount and it has been revealed that the best performance was achieved by using methanol/wet biomass ratio of 8:1. The effect of using various co-solvents in increasing the efficiency of the supercritical process was investigated. It has been shown that hexane was the most effective co-solvent and its optimal ratio respect to wet biomass was 6:1. The... 

    Thermochemical growth of Mn-doped CdS nanoparticles and study of luminescence evolution

    , Article Nanotechnology ; Volume 19, Issue 22 , 2008 ; 09574484 (ISSN) Marandi, M ; Taghavinia, N ; Sedaghat, Z ; Iraji Zad, A ; Mahdavi, S. M ; Sharif University of Technology
    We report a new method of growing Mn-doped CdS (CdS:Mn) nanoparticles in an aqueous solution at boiling temperature. The idea is to use precursors that react only at high temperature, in order to gain crystalline luminescent nanoparticles. CdSO4, Mn(NO3)2 and Na 2S2O3 were used as the precursors, and thioglycerol was employed as the capping agent and also the reaction catalyst. Na2S2O3 is thermally sensitive and it releases S2- ions upon heating. The CdS:Mn nanoparticles obtained are about 4 nm in size and show both cubic and hexagonal crystalline phases with a ratio of 35% to 65%. The luminescence of nanoparticles contains a peak at 580 nm, which is related to Mn2+ ions. Prolonged reaction... 

    The olfactory bulb modulates entorhinal cortex oscillations during spatial working memory

    , Article Journal of Physiological Sciences ; Volume 71, Issue 1 , 2021 ; 18806546 (ISSN) Salimi, M ; Tabasi, F ; Nazari, M ; Ghazvineh, S ; Salimi, A ; Jamaati, H ; Raoufy, M. R ; Sharif University of Technology
    BioMed Central Ltd  2021
    Cognitive functions such as working memory require integrated activity among different brain regions. Notably, entorhinal cortex (EC) activity is associated with the successful working memory task. Olfactory bulb (OB) oscillations are known as rhythms that modulate rhythmic activity in widespread brain regions during cognitive tasks. Since the OB is structurally connected to the EC, we hypothesized that OB could modulate EC activity during working memory performance. Herein, we explored OB–EC functional connectivity during spatial working memory performance by simultaneous recording local field potentials when rats performed a Y-maze task. Our results showed that the coherence of delta,...