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    Online analysis of local field potentials for seizure detection in freely moving rats

    , Article Iranian Journal of Basic Medical Sciences ; Volume 23, Issue 2 , 2020 , Pages 173-177 Zare, M ; Nazari, M ; Shojaei, A ; Raoufy, M. R ; Mirnajafi Zadeh, J ; Sharif University of Technology
    Mashhad University of Medical Sciences  2020
    Objective(s): Seizure detection during online recording of electrophysiological parameters is very important in epileptic patients. In the present study, online analysis of field potential recordings was used for detecting spontaneous seizures in epileptic animals. Materials and Methods: Epilepsy was induced in rats by pilocarpine injection. During the chronic period of the pilocarpine model, local field potential (LFP) recording was run for at least 24 hr. At the same time, video monitoring of the animals was done to determine the real time of seizure occurrence. Both power and sample entropy of LFP were used for online analysis. Results: Obtained results showed that changes in LFP power... 

    Allergic rhinitis impairs working memory in association with drop of hippocampal – Prefrontal coupling

    , Article Brain Research ; Volume 1758 , 2021 ; 00068993 (ISSN) Salimi, M ; Ghazvineh, S ; Nazari, M ; Dehdar, K ; Garousi, M ; Zare, M ; Tabasi, F ; Jamaati, H ; Salimi, A ; Barkley, V ; Mirnajafi Zadeh, J ; Raoufy, M. R ; Sharif University of Technology
    Elsevier B.V  2021
    Allergic rhinitis (AR) is a chronic inflammatory disease frequently associated with a deficit in learning and memory. Working memory is an important system for decision making and guidance, which depends on interactions between the ventral hippocampus (vHipp) and the prelimbic prefrontal cortex (plPFC). It is still unclear whether AR influences the activity and coupling of these brain areas, which consequently may impair working memory. The current study aimed to examine alterations of the vHipp-plPFC circuit in a rat model of AR. Our results show decreased working memory performance in AR animals, accompanied by a reduction of theta and gamma oscillations in plPFC. Also, AR reduces... 

    Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study

    , Article International Journal of Pharmaceutics ; Volume 592 , 2021 ; 03785173 (ISSN) Shamloo, A ; Aghababaie, Z ; Afjoul, H ; Jami, M ; Bidgoli, M. R ; Vossoughi, M ; Ramazani, A ; Kamyabhesari, K ; Sharif University of Technology
    Elsevier B.V  2021
    In this study, physically cross-linked hydrogels were developed by freezing-thawing method while different concentrations of honey were included into the hydrogels for accelerated wound healing. The hydrogel was composed of chitosan, polyvinyl alcohol (PVA), and gelatin with the ratio of 2:1:1 (v/v), respectively. Further, the effect of honey concentrations on antibacterial properties, and cell behavior was investigated. In vivo studies, including wound healing mechanism using rat model and histological analysis of section tissue samples were performed. The results illustrated that the incorporation of honey in hydrogels increased the ultimate strain of hydrogels approximately two times,... 

    Overexpression of protein kinase Mζ in the hippocampus mitigates alzheimer's disease-related cognitive deficit in rats

    , Article Brain Research Bulletin ; Volume 166 , 2021 , Pages 64-72 ; 03619230 (ISSN) Amini, N ; Roosta Azad, R ; Motamedi, F ; Mirzapour Delavar, H ; Ghasemi, S ; Aliakbari, S ; Pourbadie, H. G ; Sharif University of Technology
    Elsevier Inc  2021
    Accumulation of amyloid beta (Aβ) soluble forms in the cerebral parenchyma is the mainstream concept underlying memory deficit in the early phase of Alzheimer's disease (AD). PKMζ plays a critical role in the maintenance of long-term memory. Yet, the role of this brain-specific enzyme has not been addressed in AD. We examined the impact of hippocampal PKMζ overexpression on AD-related memory impairment in rats. Oligomeric form of Aβ (oAβ) or vehicle was bilaterally microinjected into the dorsal hippocampus of male Wistar rats under stereotaxic surgery. One week later, 2 μl of lentiviral vector (108 T.U. / ml.) encoding PKMζ genome was microinjected into the dorsal hippocampus. Seven days... 

    Fullerene C60 nanoparticle attenuates pain and tumor necrosis factor-α protein expression in the hippocampus following diabetic neuropathy in rats

    , Article Physiology and Pharmacology (Iran) ; Volume 26, Issue 4 , 2022 , Pages 451-458 ; 24765236 (ISSN) Namdar, F ; Bahrami, F ; Bahari, Z ; Ghanbari, B ; Shahyad, S ; Mohammadi, M. T ; Sharif University of Technology
    Iranian Society of Physiology and Pharmacology  2022
    Introduction: Diabetic neuropathy is a common complication of diabetes mellitus. It is associated with nerve damage due to oxidative stress and high levels of pro-inflammatory mediators. In the present study, we examined the anti-nociceptive effects of Fullerene nanoparticle, as a potent anti-oxidant, during diabetic neuropathy. Methods: Diabetes mellitus induced through injection of streptozotocin (STZ) (40 mg/kg). Four groups were used in the study as follows: the control, control+fullerene, diabetes, and diabetes +fullerene groups. All four groups received sesame oil. Treatment rats received fullerene C60 (1mg/kg/day) for 9 weeks by intra-gastric gavage. Then, cold allodynia, histology,... 

    Global mortality of snakebite envenoming between 1990 and 2019

    , Article Nature Communications ; Volume 13, Issue 1 , 2022 ; 20411723 (ISSN) Roberts, N. L. S ; Johnson, E. K ; Zeng, S. M ; Hamilton, E. B ; Abdoli, A ; Alahdab, F ; Alipour, V ; Ancuceanu, R ; Andrei, C. L ; Anvari, D ; Arabloo, J ; Ausloos, M ; Awedew, A. F ; Badiye, A. D ; Bakkannavar, S. M ; Bhalla, A ; Bhardwaj, N ; Bhardwaj, P ; Bhaumik, S ; Bijani, A ; Boloor, A ; Cai, T ; Carvalho, F ; Chu, D.-T ; Couto, R. A. S ; Dai, X ; Desta, A. A ; Do, H. T ; Earl, L ; Eftekhari, A ; Esmaeilzadeh, F ; Farzadfar, F ; Fernandes, E ; Filip, I ; Foroutan, M ; Franklin, R. C ; Gaidhane, A. M ; Gebregiorgis, B. G ; Gebremichael, B ; Ghashghaee, A ; Golechha, M ; Hamidi, S ; Haque, S. E ; Hayat, K ; Herteliu, C ; Ilesanmi, O. S ; Islam, M. M ; Jagnoor, J ; Kanchan, T ; Kapoor, N ; Khan, E. A ; Khatib, M. N ; Khundkar, R ; Krishan, K ; Kumar, G. A ; Kumar, N ; Landires, I ; Lim, S. S ; Madadin, M ; Maled, V ; Manafi, N ; Marczak, L. B ; Menezes, R. G ; Meretoja, T. J ; Miller, T. R ; Mohammadian Hafshejani, A ; Mokdad, A. H ; Monteiro, F. N. P ; Moradi, M ; Nayak, V. C ; Nguyen, C. T ; Nguyen, H. L.T ; Nuñez-Samudio, V ; Ostroff, S. M ; Padubidri, J. R ; Pham, H. Q ; Pinheiro, M ; Pirestani, M ; Quazi Syed, Z ; Rabiee, N ; Radfar, A ; Rahimi Movaghar, V ; Rao, S. J ; Rastogi, P ; Rawaf, D. L ; Rawaf, S ; Reiner, R.C., Jr ; Sahebkar, A ; Samy, A. M ; Sawhney, M ; Schwebel, D. C ; Senthilkumaran, S ; Shaikh, M. A ; Skryabin, V. Y ; Skryabina, A. A ; Soheili, A ; Stokes, M. A ; Thapar, R ; Tovani Palone, M. R ; Tran, B. X ; Travillian, R. S ; Velazquez, D. Z ; Zhang, Z. J ; Naghavi, M ; Dandona, R ; Dandona, L ; James, S. L ; Pigott, D.M ; Murray, C. J. L ; Hay, S. I ; Vos, T ; Sharif University of Technology
    Nature Research  2022
    Snakebite envenoming is an important cause of preventable death. The World Health Organization (WHO) set a goal to halve snakebite mortality by 2030. We used verbal autopsy and vital registration data to model the proportion of venomous animal deaths due to snakes by location, age, year, and sex, and applied these proportions to venomous animal contact mortality estimates from the Global Burden of Disease 2019 study. In 2019, 63,400 people (95% uncertainty interval 38,900–78,600) died globally from snakebites, which was equal to an age-standardized mortality rate (ASMR) of 0.8 deaths (0.5–1.0) per 100,000 and represents a 36% (2–49) decrease in ASMR since 1990. India had the greatest number... 

    Preclinical evaluation of 188 Re-HYNIC-PSMA as a novel therapeutic agent

    , Article Journal of Radioanalytical and Nuclear Chemistry ; Volume 331, Issue 2 , 2022 , Pages 841-849 ; 02365731 (ISSN) Hadisi, M ; Vosoughi, N ; Yousefnia, H ; Bahrami-Samani, A ; Zolghadri, S ; Vosoughi, S ; Alirezapour, B ; Sharif University of Technology
    Springer Science and Business Media B.V  2022
    In this study, optimized preparation, quality control, cell assessments and biostribution of 188Re-HYNIC-PSMA in normal rats and tumor bearing mice are studied. Optimized conditions for radiolabeling were evaluated and radiochemical purity (> 99%) was investigated using ITLC and HPLC methods. 188Re-HYNIC-PSMA was stable both in PBS (4 °C) and in human serum (37 °C) even after 48 h. The results showed the complex was cleared from the blood very fast via urinary track. This new radiolabeled compound has a high potential to be considered as an agent for the treatment of patients with PSMA expressing tumors however more biological studies are still needed. © 2022, Akadémiai Kiadó, Budapest,... 

    Long-term investigation on the phase stability, magnetic behavior, toxicity, and MRI characteristics of superparamagnetic Fe/Fe-oxide core/shell nanoparticles

    , Article International Journal of Pharmaceutics ; Volume 439, Issue 1-2 , 2012 , Pages 28-40 ; 03785173 (ISSN) Masoudi, A ; Madaah Hosseini, H. R ; Seyed Reyhani, S. M ; Shokrgozar, M. A ; Oghabian, M. A ; Ahmadi, R ; Sharif University of Technology
    To efficiently enhance the contrast obtaining from magnetic resonance imaging (MRI), pharmaceutical grade colloidal dispersions of PEG coated iron-based nanoparticles were prepared and compared to conventional pure iron oxide contrast agent. In this study, we synthesized ∼14 nm iron nanoparticles via NaBH4 reduction of iron(III) chloride in an aqueous medium. The resulting nanoparticles were further oxidized by two different methods via (CH3)3NO oxygen transferring agent and exposure to oxygen flow. XRD and electron microscopy analyses confirmed the formation of a second layer on the surface of α-Fe core. As magnetic measurements and Mössbauer spectra of 4-months post prepared nanoparticles... 

    The effect of poly(ethylene glycol) coating on colloidal stability of superparamagnetic iron oxide nanoparticles as potential MRI contrast agent

    , Article International Journal of Pharmaceutics ; Volume 433, Issue 1-2 , 2012 , Pages 129-141 ; 03785173 (ISSN) Masoudi, A ; Madaah Hosseini, H. R ; Shokrgozar, M. A ; Ahmadi, R ; Oghabian, M. A ; Sharif University of Technology
    Superparamganetic iron oxide-based contrast agents in magnetic resonance imaging (MRI) have offered new possibility for early detection of lymph nodes and their metastases. According to important role of nanoparticle size in biodistribution, magnetite nanoparticles coated with different polyethylene glycol (PEG) concentrations up to 10/1 PEG/iron oxide weight ratio in an ex situ manner. To predict the PEG-coated nanoparticle behavior in biological media, such as blood stream or tissue, colloidal stability evaluation was performed to estimate the coating endurance in different conditions. Accordingly, optical absorbance measurements were conducted in solutions with different values of pH and... 

    Role of endurance training in preventing pathological hypertrophy via large tumor suppressor (LATS) changes

    , Article Iranian Heart Journal ; Volume 20, Issue 3 , 2019 , Pages 52-59 ; 17357306 (ISSN) Tabrizi, A ; Soori, R ; Choobineh, S ; Gholipour, M ; Sharif University of Technology
    Iranian Heart Association  2019
    Background: One of the negative effects of cardiac sympathetic hyperactivity is pathologic hypertrophy. Recent studies have indicated that large tumor suppressor (LATS) is one of the molecules which play a critical role in cardiomyocyte apoptosis. Considering the preventive role of exercise training, we evaluated the effects of endurance training on LATS gene expression and its upstream pathway in the present study. Methods: Eighteen male Wistar rats were randomly divided into 2 groups: Endurance and control. Endurance training was performed for 8 weeks, 1 hour per day, and 6 days per week on the treadmill at a 15° inclination. Pathologic hypertrophy was induced with the injection of 3... 

    Intravenous laser wavelength radiation effect on LCAT, PON1, catalase, and FRAP in diabetic rats

    , Article Lasers in Medical Science ; Volume 35, Issue 1 , 2020 , Pages 131-138 Amjadi, A ; Mirmiranpour, H ; Sobhani, S. O ; Moazami Goudarzi, N ; Sharif University of Technology
    Springer  2020
    The main purpose of this study is to evaluate the effect of intravenous irradiation of different low-level laser wavelengths on the activity of lecithin-cholesterol acyltransferase (LCAT), paraoxonase (PON1), catalase, and ferric reducing ability of plasma (FRAP) in diabetic rats. First, diabetes was induced in rats using streptozotocin (STZ). Enzymes’ activity was measured in the blood samples and compared before and after intravenous laser blood irradiation. We used four continuous-wave lasers—IR (λ = 808 nm), Red (λ = 638 nm), Green (λ = 532 nm), and Blue (λ = 450 nm)—to compare the wavelength’s effect on different enzymes’ activity. Laser power was fixed at 0.01 mW and laser energy was... 

    The role of hippo signaling pathway in physiological cardiac hypertrophy

    , Article BioImpacts ; Volume 10, Issue 4 , 2020 , Pages 251-257 Gholipour, M ; Tabrizi, A ; Sharif University of Technology
    Tabriz University of Medical Sciences  2020
    Introduction: The role of Hippo signaling pathway, which was identified by genetic studies as a key regulator for tissue growth and organ size, in promoting physiological cardiac hypertrophy has not been investigated. Methods: Fourteen male Wistar rats were randomly assigned to the exercise and control groups. The exercise group ran 1 hour per day, 5 days/week, at about 65%-75% VO2max on the motor-driven treadmill with 15ºslope, and the control group ran 15 min/d, 2 days/ week at 9 m/min (0ºinclination), throughout the eight-week experimental period. Forty-eight hours after the last session, hearts were dissected and left ventricles were weighed and stored for subsequent RT-PCR analysis.... 

    Type V collagen in scar tissue regulates the size of scar after heart injury

    , Article Cell ; Volume 182, Issue 3 , 2020 , Pages 545-562.e23 Yokota, T ; McCourt, J ; Ma, F ; Ren, S ; Li, S ; Kim, T. H ; Kurmangaliyev, Y. Z ; Nasiri, R ; Ahadian, S ; Nguyen, T ; Tan, X. H. M ; Zhou, Y ; Wu, R ; Rodriguez, A ; Cohn, W ; Wang, Y ; Whitelegge, J ; Ryazantsev, S ; Khademhosseini, A ; Teitell, M. A ; Chiou, P. Y ; Birk, D. E ; Rowat, A. C ; Crosbie, R. H ; Pellegrini, M ; Seldin, M ; Lusis, A. J ; Deb, A ; Sharif University of Technology
    Cell Press  2020
    Scar tissue size following myocardial infarction is an independent predictor of cardiovascular outcomes, yet little is known about factors regulating scar size. We demonstrate that collagen V, a minor constituent of heart scars, regulates the size of heart scars after ischemic injury. Depletion of collagen V led to a paradoxical increase in post-infarction scar size with worsening of heart function. A systems genetics approach across 100 in-bred strains of mice demonstrated that collagen V is a critical driver of postinjury heart function. We show that collagen V deficiency alters the mechanical properties of scar tissue, and altered reciprocal feedback between matrix and cells induces... 

    New proline, alanine, serine repeat sequence for pharmacokinetic enhancement of anti-vegf single-domain antibody

    , Article Journal of Pharmacology and Experimental Therapeutics ; Volume 375, Issue 1 , July , 2020 , Pages 69-75 Khodabakhsh, F ; Salimian, M ; Mehdizadeh, A ; Khosravy, M. S ; Vafabakhsh, A ; Karami, E ; Cohan, R. A ; Sharif University of Technology
    American Society for Pharmacology and Experimental Therapy  2020
    Therapeutic fragmented antibodies show a poor pharmacokinetic profile that leads to frequent high-dose administration. In the current study, for the first time, a novel proline, alanine, serine (PAS) repeat sequence called PAS#208 was designed to extend the plasma half-life of a nanosized anti-vascular endothelial growth factor-A single-domain antibody. Polyacrylamide gel electrophoresis, circular dichroism, dynamic light scattering, and electrophoretic light scattering were used to assess the physicochemical properties of the newly designed PAS sequence. The effect of PAS#208 on the biologic activity of a single-domain antibody was studied using an in vitro proliferation assay. The... 

    An improved synthesis and preliminary biodistribution study of a technetium-99m-labeled2-amino-2-deoxy(thioacetyl)-D-glucose complex ([ 99mTc]-TA-DG) as a tumor imaging agent

    , Article Iranian Journal of Nuclear Medicine ; Volume 15, Issue 28 , 2007 , Pages 43-48 ; 16812824 (ISSN) Johari Daha, F ; Sadeghzadeh, M ; Charkhlooie, G ; Haghir Ebrahimabadi, K ; Saeedi, M. R ; Sharif University of Technology
    Introduction: This report describes the synthesis of 2-Amino-2-deoxy(S- benzoylthioacetyl)-D-glucose (S-Bz-TA-DG), radiolabeled with [ 99mTc(CO)3(OH2)3]+ complex with a procedure including deprotection of the benzoyl group, characterization by HPLC using a C18 reverse phase column and preliminary biodistribution study in normal mice. Methods: [99mTc(CO) 3(H2O)3]+ complex was used to label TA-DG with 99mTc. This complex was prepared using up to 46 mCi of Na99mTcO4 in 1mL saline. The radiochemical purity (>95%) was determined by TLC in normal saline solution as the mobile phase. Radio-HPLC analysis of [99mTc]-(TA-DG) at pH=9.5-10, revealed that labeling with 99mTc resulted in the formation of... 

    Quantitative in vivo microsampling for pharmacokinetic studies based on an integrated solid-phase microextraction system

    , Article Analytical Chemistry ; Volume 79, Issue 12 , 2007 , Pages 4507-4513 ; 00032700 (ISSN) Zhang, X ; Eshaghi, A ; Musteata, F. M ; Ouyang, G ; Pawliszyn, J ; Sharif University of Technology
    An integrated microsampling approach based on solid-phase microextraction (SPME) was developed to provide a complete solution to highly efficient and accurate pharmacokinetic studies. The microsampling system included SPME probes that are made of poly(ethylene glycol) (PEG) and C18-bonded silica, a fast and efficient sampling strategy with accurate kinetic calibration, and a high-throughput desorption device based on a modified 96-well plate. The sampling system greatly improved the quantitative capability of SPME in two ways. First, the use of the C18-bonded silica/PEG fibers minimized the competition effect from analogues of the target analytes in a complicated sample matrix such as blood... 

    Hyperthermia of breast cancer tumor using graphene oxide-cobalt ferrite magnetic nanoparticles in mice

    , Article Journal of Drug Delivery Science and Technology ; Volume 65 , 2021 ; 17732247 (ISSN) Hatamie, S ; Balasi, Z. M ; Ahadian, M. M ; Mortezazadeh, T ; Shams, F ; Hosseinzadeh, S ; Sharif University of Technology
    Editions de Sante  2021
    Herein, the graphene oxide (GO)/cobalt ferrite nanoparticles were used to apply the heat treatment on the breast cancer cell line of MCF7. The synthesized nanoparticles were evaluated before in vitro and in vivo studies, using transmission electron microscopy (TEM), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), X-ray photoelectron spectroscopy (XPS), thermal property and relaxivity measurement. The nanoparticles showed a diameter of 5 nm with the ferrimagnetic property. Also, the nanoparticles were well distributed on the GO nanosheets. The related peaks of cobalt ferrite nanoparticles were approved by using XRD and XPS assays. During the in vitro investigations, IC50 with... 

    Novel force–displacement control passive finite element models of the spine to simulate intact and pathological conditions; comparisons with traditional passive and detailed musculoskeletal models

    , Article Journal of Biomechanics ; Volume 141 , 2022 ; 00219290 (ISSN) Abbasi-Ghiri, A ; Ebrahimkhani, M ; Arjmand, N ; Sharif University of Technology
    Elsevier Ltd  2022
    Passive finite element (FE) models of the spine are commonly used to simulate intact and various pre- and postoperative pathological conditions. Being devoid of muscles, these traditional models are driven by simplistic loading scenarios, e.g., a constant moment and compressive follower load (FL) that do not properly mimic the complex in vivo loading condition under muscle exertions. We aim to develop novel passive FE models that are driven by more realistic yet simple loading scenarios, i.e., in vivo vertebral rotations and pathological-condition dependent FLs (estimated based on detailed musculoskeletal finite element (MS-FE) models). In these novel force–displacement control FE models,... 

    Oral administration of lithium chloride ameliorate spinal cord injury-induced hyperalgesia in male rats

    , Article PharmaNutrition ; Volume 21 , 2022 ; 22134344 (ISSN) Rahimi, G ; Mirsadeghi, S ; Rahmani, S ; Izadi, A ; Ghodsi, Z ; Ghodsi, S. M ; Rahimi Movaghar, V ; Kiani, S ; Sharif University of Technology
    Elsevier B.V  2022
    Background: Numerous studies have described the neuroprotective effect of lithium in spinal cord injury in addition to its ameliorative impact on pain sensation. In the present study, we aim to examine the efficacy of 85 mg/kg as well as 50 mg/kg dosage of the lithium chloride (LiCl) through oral consumption in spinal cord injured rats and their effect on gene expression of three candidate genes, corresponding to the hyper-sensitization. Methods: Adult Wistar (male) rats were divided into four experimental groups: control; oral administration of LiCl with 85 mg/kg and 50 mg/kg dosage; and 10 % sucrose receiver as the vehicle. BBB and heat plantar tests were performed weekly throughout four... 

    A novel formulation of simvastatin nanoemulsion gel for infected wound therapy: In vitro and in vivo assessment

    , Article Journal of Drug Delivery Science and Technology ; Volume 72 , 2022 ; 17732247 (ISSN) Amoozegar, H ; Ghaffari, A ; Keramati, M ; Ahmadi, S ; Dizaji, S ; Moayer, F ; Akbarzadeh, I ; Abazari, M ; razzaghi abyaneh, M ; Bakhshandeh, H ; Sharif University of Technology
    Editions de Sante  2022
    Simvastatin, a well-known antihyperlipidemic drug, has antibacterial activity against a broad range of bacteria, especially Staphylococcus aureus. In present study, a nanoemulsion gel-based formulation containing Simvastatin was developed for infected wound therapy. Therefore, different formulations of Simvastatin nanoemulsion were prepared. Based on droplet size, polydispersity index and zeta potential, the best nanoemulsion formulation containing Simvastatin was selected for development of nanoemulsion gel formulation of drug using carbomer 934 as gelling agent. Thermodynamic stability of Simvastatin nanoemulsion was assessed at different conditions. The in vitro antibacterial activity...