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    Optimization, physicochemical characterization, and antimicrobial activity of a novel simvastatin nano-niosomal gel against E. coli and S. aureus

    , Article Chemistry and Physics of Lipids ; Volume 234 , 2021 ; 00093084 (ISSN) Akbarzadeh, I ; Keramati, M ; Azadi, A ; Afzali, E ; Shahbazi, R ; chiani, M ; Norouzian, D ; Bakhshandeh, H ; Sharif University of Technology
    Elsevier Ireland Ltd  2021
    Abstract
    Niosomes, as a kind of drug delivery system, is widely used for the topical delivery of lipophilic drugs. Optimization of niosomes plays an essential role in enhancing their therapeutic efficiencies. This study aims to prepare an optimized niosomal formulation of simvastatin (nSIM), a lipophilic member of statins, through the experiment (Response Surface methodology). Optimized niosomes were characterized in size, polydispersity index (PDI), entrapment efficiency (EE), stability, releasing pattern, and antimicrobial activity. The different molar ratio of surfactant and cholesterol were applied to prepare various formulation of simvastatin loaded niosome. Mean particle size and size... 

    Optimization, physicochemical characterization, and antimicrobial activity of a novel simvastatin nano-niosomal gel against E. coli and S. aureus

    , Article Chemistry and Physics of Lipids ; Volume 234 , 2021 ; 00093084 (ISSN) Akbarzadeh, I ; Keramati, M ; Azadi, A ; Afzali, E ; Shahbazi, R ; chiani, M ; Norouzian, D ; Bakhshandeh, H ; Sharif University of Technology
    Elsevier Ireland Ltd  2021
    Abstract
    Niosomes, as a kind of drug delivery system, is widely used for the topical delivery of lipophilic drugs. Optimization of niosomes plays an essential role in enhancing their therapeutic efficiencies. This study aims to prepare an optimized niosomal formulation of simvastatin (nSIM), a lipophilic member of statins, through the experiment (Response Surface methodology). Optimized niosomes were characterized in size, polydispersity index (PDI), entrapment efficiency (EE), stability, releasing pattern, and antimicrobial activity. The different molar ratio of surfactant and cholesterol were applied to prepare various formulation of simvastatin loaded niosome. Mean particle size and size... 

    Electrochemical and computational studies of bio-mimicked Ti3C2Tx MXene-based sensor with multivalent interface

    , Article Journal of Colloid and Interface Science ; Volume 623 , 2022 , Pages 1063-1074 ; 00219797 (ISSN) Ranjbar, S ; Ashari Astani, N ; Atabay, M ; Naseri, N ; Esfandiar, A ; Reza Ejtehadi, M ; Sharif University of Technology
    Academic Press Inc  2022
    Abstract
    Two-dimensional MXenes are the newly emerging family of nanomaterials with competitive performance for nano-device development. Surface functional groups and abundant binding sites make these materials ideal candidates for sensor applications. Herein, we report the successful fabrication of a MXene-based nano-bio device for capturing, sensing, and filtering the Escherichia coli (E. coli) bacteria. Mannose carbohydrate, which binds strongly to E.coli's fimH protein via glucan multivalent interactions, is used as the bio-receptor element. MXene's structure was engineered to guarantee efficient E. coli capturing without mannose detachment. Electrochemical impedance spectroscopy (EIS) and cyclic... 

    Alginate-based multifunctional films incorporated with sulfur quantum dots for active packaging applications

    , Article Colloids and Surfaces B: Biointerfaces ; Volume 215 , 2022 ; 09277765 (ISSN) Riahi, Z ; Priyadarshi, R ; Rhim, J. W ; Lotfali, E ; Bagheri, R ; Pircheraghi, G ; Sharif University of Technology
    Elsevier B.V  2022
    Abstract
    Sulfur quantum dots (SQDs) were fabricated using a facile hydrothermal method and used for the preparation of functional food packaging film and compared the properties with other sulfur-based fillers like elemental sulfur (ES) and sulfur nanoparticles (SNP). The SQDs have an average size of 5.3 nm and were very stable in aqueous suspension. Unlike other sulfur-based fillers, the SQD showed high antioxidant, antibacterial and antifungal activity, but no cytotoxicity was found for L929 mouse fibroblasts even after long-term exposure of 48 h. When sulfur-based fillers were added to the alginate film, SQD was more evenly dispersed in the polymer matrix than SNP and ES. The addition of SQD to... 

    Structural characterization of a rhamnolipid-type biosurfactant produced by Pseudomonas aeruginosa MR01: Enhancement of di-rhamnolipid proportion using gamma irradiation

    , Article Colloids and Surfaces B: Biointerfaces ; Volume 81, Issue 2 , 2010 , Pages 397-405 ; 09277765 (ISSN) Lotfabad, T. B ; Abassi, H ; Ahmadkhaniha, R ; Roostaazad, R ; Masoomi, F ; Zahiri, H. S ; Ahmadian, G ; Vali, H ; Noghabi, K. A ; Sharif University of Technology
    2010
    Abstract
    We previously reported that MR01, an indigenous strain of Pseudomonas aeruginosa, was able to produce a rhamnolipid-type biosurfactant. Here, we attempted to define the structural properties of this natural product. The analysis of the extracted biosurfactant by thin-layer chromatography (TLC) revealed the presence of two compounds corresponding to those of authentic mono- and di-rhamnolipid. The identity of two structurally distinguished rhamnolipids was confirmed by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. Liquid chromatography/mass spectrometry (LC/MS) of extracted biosurfactant revealed up to seventeen different rhamnolipid congeners. Further quantification showed... 

    Examination of chondroitinase ABC I immobilization onto dextran-coated Fe3O4 nanoparticles and its in-vitro release

    , Article Journal of Biotechnology ; Volume 309 , 2020 , Pages 131-141 Askaripour, H ; Vossoughi, M ; Khajeh, K ; Alemzadeh, I ; Sharif University of Technology
    Elsevier B.V  2020
    Abstract
    Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature. In this study, cABC I enzyme was immobilized on the dextran-coated Fe3O4 nanoparticles through physical adsorption to improve the thermal stability. The nanoparticles were characterized using XRD, SEM, VSM, and FTIR analyses. Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the... 

    Biosynthesis of copper oxide nanoparticles with potential biomedical applications

    , Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 3983-3999 Rabiee, N ; Bagherzadeh, M ; Kiani, M ; Ghadiri, A. M ; Etessamifar, F ; Jaberizadeh, A. H ; Shakeri, A ; Sharif University of Technology
    Dove Medical Press Ltd  2020
    Abstract
    Introduction: In recent years, the use of cost-effective, multifunctional, environmentally friendly and simple prepared nanomaterials/nanoparticles have been emerged considerably. In this manner, different synthesizing methods were reported and optimized, but there is still lack of a comprehensive method with multifunctional properties. Materials and Methods: In this study, we aim to synthesis the copper oxide nanoparticles using Achillea millefolium leaf extracts for the first time. Catalytic activity was investigated by in situ azide alkyne cycloaddition click and also A3 coupling reaction, and optimized in terms of temperature, solvent, and time of the reaction. Furthermore, the... 

    An innovative, highly stable Ag/ZIF-67@GO nanocomposite with exceptional peroxymonosulfate (PMS) activation efficacy, for the destruction of chemical and microbiological contaminants under visible light

    , Article Journal of Hazardous Materials ; Volume 413 , 2021 ; 03043894 (ISSN) Kohantorabi, M ; Giannakis, S ; Moussavi, G ; Bensimon, M ; Gholami, M. R ; Pulgarin, C ; Sharif University of Technology
    Elsevier B.V  2021
    Abstract
    In this work, Ag nanoparticles were loaded on ZIF-67 covered by graphene oxide (Ag/ZIF-67@GO), and its catalytic performance was studied for the heterogeneous activation of peroxymonosulfate (PMS) under visible-light. The catalyst surface morphology and structure were analyzed by FT-IR, XRD, XPS, DRS, FE-SEM, EDX, TEM, BET, ICP-AES and TGA analysis. The efficacy of PMS activation by the Ag/ZIF-67@GO under visible light was assessed by phenol degradation and E. coli inactivation. Phenol was completely degraded within 30 min by HO•, SO4•− and O2•− generated through the photocatalytic PMS activation. In addition, total E. coli inactivation was attained in 15 min that confirmed the highly... 

    Electrospun Ag-decorated reduced GO-graft-chitosan composite nanofibers with visible light photocatalytic activity for antibacterial performance

    , Article Chemosphere ; Volume 299 , 2022 ; 00456535 (ISSN) Asgari, S ; Mohammadi Ziarani, G ; Badiei, A ; Setayeshmehr, M ; Kiani, M ; Pourjavadi, A ; Sharif University of Technology
    Elsevier Ltd  2022
    Abstract
    The treatment of water contaminated by bacteria is becoming a necessity. The nanomaterials possessing both intrinsic antibacterial properties and photocatalytic activity are excellent candidates for water disinfection. The powdered form of nanomaterials can be aggregated while embedding the nanomaterials into the NFs can overcome the limitation and enhance the photocatalytic activity and transition from UV-light to visiblelight. Here, graphene oxide (GO) was synthesized, grafted to chitosan, and decorated with silver nanoparticles (Ag NPs) to produce Ag-decorated reduced GO-graft-Chitosan (AGC) NPs. The blends of polyacrylonitrile (PAN) and AGC NPs were prepared in various concentrations of... 

    Development of HAp/GO/Ag coating on 316 LVM implant for medical applications

    , Article Journal of the Mechanical Behavior of Biomedical Materials ; Volume 126 , 2022 ; 17516161 (ISSN) Ahmadi, R ; Izanloo, S ; Sharif University of Technology
    Elsevier Ltd  2022
    Abstract
    In this study, antibacterial activity, biocompatibility, and corrosion resistance of 316 LVM implants were improved using the development of HAp/GO/Ag nanocomposite coatings by the dip-coating method. The XRD and FTIR results confirmed the synthesis of HAp/GO/Ag nanocomposites. HAp/Ag nanoparticles (68 nm) bound to epoxy, hydroxyl, and carboxyl functional groups on GO sheets (size of GO sheets varies from 255 to 1480 nm) by electrostatic interaction. FESEM images showed that HAp/GO/Ag coatings had higher density and fewer micro-cracks than pure HAp coatings. In addition, HAp/GO/Ag coatings showed optimized nano-hardness (4.5 GPa) and elasticity modulus (123 GPa). The results of... 

    Design and fabrication of an electrochemical aptasensor using Au nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite for rapid and sensitive detection of Staphylococcus aureus

    , Article Bioelectrochemistry ; Volume 123 , 2018 , Pages 70-76 ; 15675394 (ISSN) Ranjbar, S ; Shahrokhian, S ; Sharif University of Technology
    Elsevier B.V  2018
    Abstract
    Since that pathogenic bacteria are major threats to human health, this paper describes the fabrication of an effective and durable sensing platform based on gold nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite (AuNPs/CNPs/CNFs) at the surface of glassy carbon electrode for sensitive and selective detection of Staphylococcus aureus (S. aureus). The AuNPs/CNPs/CNFs nanocomposite with the high surface area, excellent conductivity, and good biocompatibility was used for self-assembled of the thiolated specific S. aureus aptamer as a sensing element. The surface morphology of AuNPs/CNPs/CNFs nanocomposite was characterized with field emission scanning electron microscopy...