Search for: escherichia-coli
Total 125 records
Characterization of a microfluidic microbial fuel cell as a power generator based on a nickel electrode, Article Biosensors and Bioelectronics ; Volume 79 , 2016 , Pages 327-333 ; 09565663 (ISSN) ; Yaghmaei, S ; Sharif University of Technology
Elsevier Ltd 2016
This study reports the fabrication of a microfluidic microbial fuel cell (MFC) using nickel as a novel alternative for conventional electrodes and a non-phatogenic strain of Escherichia coli as the biocatalyst. The feasibility of a microfluidic MFC as an efficient power generator for production of bioelectricity from glucose and urea as organic substrates in human blood and urine for implantable medical devices (IMDs) was investigated. A maximum open circuit potential of 459mV was achieved for the batch-fed microfluidic MFC. During continuous mode operation, a maximum power density of 104Wm-3 was obtained with nutrient broth. For the glucose-fed microfluidic MFC, the maximum power density of...
High antimicrobial activity and low human cell cytotoxicity of core-shell magnetic nanoparticles functionalized with an Antimicrobial Peptide, Article ACS Applied Materials and Interfaces ; Volume 8, Issue 18 , 2016 , Pages 11366-11378 ; 19448244 (ISSN) ; Rai, A ; Pinto, S ; Evangelista, M ; Cardoso, R. M. S ; Paulo, C ; Carvalheiro, T ; Paiva, A ; Imani, M ; Simchi, A ; Durães, L ; Portugal, A ; Ferreira, L ; Sharif University of Technology
American Chemical Society 2016
Superparamagnetic iron oxide nanoparticles (SPIONs) functionalized with antimicrobial agents are promising infection-targeted therapeutic platforms when coupled with external magnetic stimuli. These antimicrobial nanoparticles (NPs) may offer advantages in fighting intracellular pathogens as well as biomaterial-associated infections. This requires the development of NPs with high antimicrobial activity without interfering with the biology of mammalian cells. Here, we report the preparation of biocompatible antimicrobial SPION@gold core-shell NPs based on covalent immobilization of the antimicrobial peptide (AMP) cecropin melittin (CM) (the conjugate is named AMP-NP). The minimal inhibitory...
Photocatalytic reduction of graphene oxide nanosheets on TiO2 thin film for photoinactivation of bacteria in solar light irradiation, Article Journal of Physical Chemistry C ; Volume 113, Issue 47 , 2009 , Pages 20214-20220 ; 19327447 (ISSN) ; Ghaderi, E ; Sharif University of Technology
Graphene oxide platelets synthesized by using a chemical exfoliation method were deposited on anatase TiO2 thin films. Postannealing of the graphene oxide/TiO2 thin films at 400 °C in air resulted in partial formation of a Ti-C bond between the platelets and their beneath thin film. By using atomic force microscopy and X-ray photoelectron spectroscopy analyses, UV-visible light-induced photocatalytic reduction of the graphene oxide platelets of the annealed graphene oxide/TiO2. thin films immersed in ethanol was studied for the different irradiation times. After 4 h of photocatalytic reduction, the vertical space between the platelets decreased from about 1.1 to less than 0.8 nm and the...
Physicochemical and antibacterial properties of chitosan-polyvinylpyrrolidone films containing self-organized graphene oxide nanolayers, Article Journal of Applied Polymer Science ; Volume 133, Issue 11 , 2016 ; 00218995 (ISSN) ; Ostadhossein, F ; Simchi, A ; Sharif University of Technology
John Wiley and Sons Inc 2016
Chitosan films have a great potential to be used for wound dressing and food-packaging applications if their physicochemical properties including water vapor permeability, optical transparency, and hydrophilicity are tailored to practical demands. To address these points, in this study, chitosan (CS) was combined with polyvinylpyrrolidone (PVP) and graphene oxide (GO) nanosheets (with a thickness of ∼1 nm and lateral dimensions of few micrometers). Flexible and transparent films with a high antibacterial capacity were prepared by solvent casting methods. By controlling the evaporation rate of the utilized solvent (1 vol % acidic acid in deionized water), self-organization of GO in the...
Chemical composition and antibacterial activity of dracocephalum kotschyi essential oil obtained by microwave extraction and hydrodistillation, Article International Journal of Food Properties ; Volume 20 , 2017 , Pages S306-S315 ; 10942912 (ISSN) ; Mirzania, F ; Sonboli, A ; Matloubi Moghaddam, F ; Sharif University of Technology
Dracocephalum kotschyi essential oils obtained by hydrodistillation (HD), microwave-assisted hydrodistillation (MAHD), and solvent-free microwave extraction (SFME) were investigated by GC-FID (Gas Chromatography-Flame Ionization Detector) and GC-MS (Gas Chromatography-Mass Spectrometry). The percentage of oxygenated compounds was significantly increased from 62.52% in HD to 76.47% in MAHD, and 84.52% in SFME. Conversely, the monoterpene hydrocarbons were decreased from 30.84% in HD to 13.71% in MAHD, and 5.85% in SFME. The main compound in the essential oil obtained by HD is limonene, which accounted for more than 30% of the oil, while the percentage of this compound was reduced to 9.52% in...
Article Materials Research Innovations ; Volume 21, Issue 7 , 2017 , Pages 434-438 ; 14328917 (ISSN) ; Padervand, M ; Vosoughi, M ; Roosta Azad, R ; Sharif University of Technology
CuO flower-like microcrystals, prepared by a facile template-free thermal method, showed incredible photocatalytic activity towards degradation of Acid Blue 92 (AB92), an organic wastewater, Escherichia coli and Staphylococcus aureus pathogenic bacteria under visible light. The products were well characterised by X-ray diffraction (XRD), Brunauer–Emmett–Teller (BET), scanning electron microscope (SEM), Fourier transform infrared (FTIR), photoluminescence spectroscopy (PL) and diffuse reflectance spectra (DRS) analysis methods. The XRD pattern of the products well confirmed the formation of copper oxide crystalline phase without any other impurities. The results of the photocatalytic...
Article Micro and Nano Letters ; Volume 12, Issue 9 , 2017 , Pages 647-651 ; 17500443 (ISSN) ; Yazdanpanah, A ; Abrishamkar, A ; Moztarzadeh, F ; Ramedani, A ; Pouraghaie, S ; Shirinzadeh, H ; Samadikuchaksaraei, A ; Chauhan, N. P. S ; Hopkinson, L ; Sefat, F ; Mozafari, M ; Sharif University of Technology
The most important issue during synthesis of nanoparticles (NPs) is to avoid particle agglomeration and adhesion. There have been several attempts to use special substances such as organic surfactants, polymers and stable ligands for this purpose. In this study, silver NPs were synthesised with and without gelatin macromolecules, as a green natural biopolymer, which resulted in NPs with varying shapes and sizes. The effect of morphological characteristics on the antibacterial and antifungal properties of the synthesised NPs were studied, by comparing Gram-negative (Escherichia coli) versus Gram-positive (Staphylococcus aureus) bacteria as well as fungi (Candida albicans) by calculation of...
Article Channels ; Volume 11, Issue 3 , 2017 , Pages 209-223 ; 19336950 (ISSN) ; Bavi, O ; Vossoughi, M ; Naghdabadi, R ; Hill, A. P ; Martinac, B ; Jamali, Y ; Sharif University of Technology
Taylor and Francis Inc 2017
Gating of mechanosensitive (MS) channels is driven by a hierarchical cascade of movements and deformations of transmembrane helices in response to bilayer tension. Determining the intrinsic mechanical properties of the individual transmembrane helices is therefore central to understanding the intricacies of the gating mechanism of MS channels. We used a constant-force steered molecular dynamics (SMD) approach to perform unidirectional pulling tests on all the helices of MscL in M. tuberculosis and E. coli homologs. Using this method, we could overcome the issues encountered with the commonly used constant-velocity SMD simulations, such as low mechanical stability of the helix during...
Dynamical analysis of microfluidic microbial electrolysis cell via integrated experimental investigation and mathematical modeling, Article Electrochimica Acta ; Volume 227 , 2017 , Pages 317-329 ; 00134686 (ISSN) ; Yaghmaei, S ; Sharif University of Technology
Elsevier Ltd 2017
The present study deals with the feasibility of a microfluidic microbial electrolysis cell (MEC) as an efficient biohydrogen generator for medical usage for the first time. The evaluation of nickel in microfluidic MEC as an alternative for conventional electrodes indicates successful performance in the improvement of bioenergy production. The maximum biohydrogen production rate and produced power density of 2.2 μW cm−2 and 1.4 μl H2 μl substrate−1 day−1 were obtained, respectively. It is considered a promising technology for medical usage due to the following factors: significant biohydrogen generation, low consumption of expensive materials, simple construction, and utilization of human...
Article Chemical Engineering Communications ; Volume 204, Issue 2 , 2017 , Pages 216-220 ; 00986445 (ISSN) ; Alemzadeh, I ; Sharif University of Technology
Taylor and Francis Ltd 2017
In this study, anti-leukemic enzyme L-asparaginase (E.C.22.214.171.124) from Escherichia coli ATCC 11303 was modified by the microencapsulation technique onto calcium alginate beads. Using response surface methodology (RSM), a three-level full factorial design, the values of concentration of sodium alginate, concentration of calcium chloride, and enzyme loading were investigated to obtain the highest residual L-asparaginase (L-ASNase) activity % (immobilized enzyme activity/free enzyme activity). The effects of the studied factors on immobilization were evaluated The predicted values by the model were close to the experimental values, indicating suitability of the model. The results presented that...
Characterization of sol-gel derived silver/fluor-hydroxyapatite composite coatings on titanium substrate, Article Surface and Coatings Technology ; Volume 352 , 2018 , Pages 522-528 ; 02578972 (ISSN) ; Abbasi Khazaei, B ; Afshar, A ; Sharif University of Technology
Elsevier B.V 2018
A composite coating containing silver, fluoride and hydroxyapatite (Ag-FHA) was developed on titanium substrate by sol-gel method. Triethylphosphite, hydrated calcium nitrate, ammonium fluoride and silver nitrate were used respectively, as P, Ca, F and Ag precursors with a Ca:P ratio 1.67 and concentration of silver was 0.3 wt%. Coatings were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM) and atomic absorption spectrometry (AAS). Potentiodynamic polarization measurements in simulated body fluid proved corrosion protection caused by both Ag-FHA coatings. Antibacterial activity of coatings against...
Design and fabrication of an electrochemical aptasensor using Au nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite for rapid and sensitive detection of Staphylococcus aureus, Article Bioelectrochemistry ; Volume 123 , 2018 , Pages 70-76 ; 15675394 (ISSN) ; Shahrokhian, S ; Sharif University of Technology
Elsevier B.V 2018
Since that pathogenic bacteria are major threats to human health, this paper describes the fabrication of an effective and durable sensing platform based on gold nanoparticles/carbon nanoparticles/cellulose nanofibers nanocomposite (AuNPs/CNPs/CNFs) at the surface of glassy carbon electrode for sensitive and selective detection of Staphylococcus aureus (S. aureus). The AuNPs/CNPs/CNFs nanocomposite with the high surface area, excellent conductivity, and good biocompatibility was used for self-assembled of the thiolated specific S. aureus aptamer as a sensing element. The surface morphology of AuNPs/CNPs/CNFs nanocomposite was characterized with field emission scanning electron microscopy...
Silver oxide nanoparticles-decorated tantala nanotubes for enhanced antibacterial activity and osseointegration of Ti6Al4V, Article Materials and Design ; Volume 154 , 2018 , Pages 28-40 ; 02641275 (ISSN) ; Dabbagh, A ; Abdul Razak, B ; Nasiri Tabrizi, B ; Hosseini, H. R. M ; Saber Samandari, S ; Abu Kasim, N. H ; Yean, L. K ; Sukiman, N. L ; Sharif University of Technology
Elsevier Ltd 2018
A long-term antibacterial activity of implants without compromising their biocompatibility is highly desirable to minimize the biomaterial-associated infections. Although tantalum oxide nanotubes (Ta2O5 NTs) are promising coating materials for orthodontics and orthopedics applications, their insufficient antibacterial activity retains vulnerability to post-implantation infections. This study aimed to endow antibacterial characteristics to the Ta2O5 NTs-coated Ti6Al4V substrates by decoration of silver oxide nanoparticles (Ag2O NPs). The well-adherent Ta2O5 NTs were grown via anodization of a pure tantalum (Ta) layer coated by physical vapor deposition (PVD) on the Ti6Al4V substrates. The...
Highly-ordered TiO2 nanotubes decorated with Ag2O nanoparticles for improved biofunctionality of Ti6Al4V, Article Surface and Coatings Technology ; Volume 349 , 2018 , Pages 1008-1017 ; 02578972 (ISSN) ; Dabbagh, A ; Abdul Razak, B ; Mahmoodian, R ; Nasiri Tabrizi, B ; Madaah Hosseini, H. R ; Saber Samandari, S ; Abu Kasim, N. H ; Abdullah, H ; Sukiman, N. L ; Sharif University of Technology
Elsevier B.V 2018
The nanotubular arrays of titanium dioxide (TiO2 NTs) have recently received considerable interest for fabrication of dental and orthopedic implants. However, their antibacterial activity requires substantial improvement for the potential infections minimization, without compromise of their biocompatibility. In this work, TiO2 NTs were developed on Ti6Al4V substrates via anodization at a constant potential of 60 V for 60 min, followed by heat treatment at 500 °C for 90 min. Physical vapor deposition (PVD) was further employed to decorate silver oxide nanoparticles (Ag2O NPs) on the nanotubular edges. The results indicated that the highly-ordered TiO2 NTs with decorated Ag2O NPs could promote...
Ternary composite of TiO2 nanotubes/Ti plates modified by g-C3N4 and SnO2 with enhanced photocatalytic activity for enhancing antibacterial and photocatalytic activity, Article Journal of Photochemistry and Photobiology B: Biology ; Volume 178 , 2018 , Pages 124-132 ; 10111344 (ISSN) ; Mohaghegh, N ; Abedini, A ; Sharif University of Technology
Elsevier B.V 2018
A series of g-C3N4-SnO2/TiO2 nanotubes/Ti plates were fabricated via simple dipping of TiO2 nanotubes/Ti in a solution containing SnCl2 and g-C3N4 nanosheets and finally annealing of the plates. Synthesized plates were characterized by various techniques. The SEM analysis revealed that the g-C3N4-SnO2 nanosheets with high physical stability have been successfully deposited onto the surface of TiO2 nanotubes/Ti plate. Photocatalytic activity was investigated using two probe chemical reactions: oxidative decomposition of acetic acid and oxidation of 2-propanol under irradiation. Antibacterial activities for Escherichia coli (E. coli) bacteria were also investigated in dark and under UV/Vis...
Effect of graphene oxide nanosheets on visible light-assisted antibacterial activity of vertically-aligned copper oxide nanowire arrays, Article Journal of Colloid and Interface Science ; Volume 521 , 2018 , Pages 119-131 ; 00219797 (ISSN) ; Ashari Astani, N ; Rahighi, R ; Tayyebi, A ; Tayebi, M ; Khezri, J ; Hashemi, E ; Rothlisberger, U ; Simchi, A ; Sharif University of Technology
Academic Press Inc 2018
In the present work, the effect of graphene oxide (GO) nanosheets on the antibacterial activity of CuO nanowire arrays under visible light irradiation is shown. A combined thermal oxidation/electrophoretic deposition technique was employed to prepare three-dimensional networks of graphene oxide nanosheets hybridized with vertically aligned CuO nanowires. With the help of standard antibacterial assays and X-ray photoelectron spectroscopy, it is shown that the light-activated antibacterial response of the hybrid material against gram-negative Escherichia coli is significantly improved as the oxide functional groups of the GO nanosheets are reduced. In order to explore the physicochemical...
Label-free and simple detection of endotoxins using a sensitive LSPR biosensor based on silver nanocolumns, Article Analytical Biochemistry ; Volume 548 , 2018 , Pages 96-101 ; 00032697 (ISSN) ; Hosseini, N ; Vossoughi, M ; Khatami, M ; Abbasian, S ; Moshaii, A ; Sharif University of Technology
Academic Press Inc 2018
This paper describes the construction of a silver-based LSPR biosensor for endotoxin detection. We used GLAD method to procure reproducible silver nanocolumns. In this work, the silver nanostructures were considerably stabilized by a SAM of MPA, and the limit of detection of biosensor was measured to be 340 pg/ml for endotoxin E. coli. Considering endotoxin B. abortus as the second type of endotoxin contamination in our target samples (HBs-ag produced in Institute Pasteur, Iran), we investigated selectivity of the biosensor in various experiments. We showed that this biosensor can selectively detect both types of endotoxins compared to other biological species. Overall, this study proposes...
Article Physica A: Statistical Mechanics and its Applications ; Volume 493 , 2018 , Pages 410-420 ; 03784371 (ISSN) ; Fatemizadeh, E ; Pezeshk, H ; Sadeghi, M ; Sharif University of Technology
Elsevier B.V 2018
Random decision making in genetic switches can be modeled as tossing a biased coin. In other word, each genetic switch can be considered as a game in which the reactive elements compete with each other to increase their molecular concentrations. The existence of a very small number of reactive element molecules has caused the neglect of effects of noise to be inevitable. Noise can lead to undesirable cell fate in cellular differentiation processes. In this paper, we study the robustness to noise in genetic switches by considering another switch to have a new gene regulatory network (GRN) in which both switches have been affected by the same noise and for this purpose, we will use Parrondo's...
A continuous flow microfluidic device based on contactless dielectrophoresis for bioparticles enrichment, Article Electrophoresis ; Volume 39, Issue 3 , 2018 , Pages 445-455 ; 01730835 (ISSN) ; Mohammadi, A. A ; Kalhor, H. R ; Sharif University of Technology
Wiley-VCH Verlag 2018
In recent years, applications of dielectrophoresis-based platforms have been recognized as effective and dependable approach to separate cells and bioparticles, suspended in different carrier fluids, based on particle size and electrical properties. In this study, a microfluidic device was fabricated by an unprecedented electrode pattern, and several experiments were performed to enrich samples including either of yeast, Escherichia coli, or latex particles. A chemical deposition-based method was employed for fabrication of microelectrodes, inducing nonuniform electric field required for dielectrophoresis-based separation. One major advantage of our employed method is low fabrication cost,...
Synthesis photocatalytic TiO2/ZnO nanocomposite and investigation through anatase, wurtzite and ZnTiO3 phases antibacterial behaviors, Article Journal of Nano Research ; Volume 51 , 2018 , Pages 69-77 ; 16625250 (ISSN) ; Ghorbani, M ; Sharif University of Technology
Trans Tech Publications Ltd 2018
Titanium dioxide is prepared by sol gel method from titanium tetraisopropoxide (TTIP) as precursor and likewise zinc oxide is prepared by sol gel method from zinc acetate dehydrate (ZAD) as precursor. The composite sols are prepared in three different molar ratios 90TiO2:10ZnO, 70TiO2:30ZnO and 50TiO2:50ZnO. Thin film deposition is carried out by dip coating technique. Crystal structure, surface morphology and photocatalytic activity of the prepared nanocomposite thin films are investigated. The antibacterial activity of the prepared nanocomposite thin film against E-coli ATCC 25922 bacteria is examined by placing the thin film in standard aqueous E-coli medium under UV light for 1, 2, 3 and...